Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free (ab213004)
Key features and details
- Mouse monoclonal [C5/473 + CD5/54/F6] to CD5 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD5 antibody [C5/473 + CD5/54/F6] - BSA and Azide free
See all CD5 primary antibodies -
Description
Mouse monoclonal [C5/473 + CD5/54/F6] to CD5 - BSA and Azide free -
Host species
Mouse -
Specificity
ab200697 does not react with granulocytes or monocytes.
-
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD5. Clone C5/473
Database link: P06127 -
Positive control
- 293T, Ramos, or MOLT4 cells; Human tonsil tissue.
-
General notes
ab213004 is a carrier free version of ab200697. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-).
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
ab2697 was purified from Bioreactor Concentrate. -
Clonality
Monoclonal -
Clone number
C5/473 + CD5/54/F6 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
for 30 minutes at RT
|
Notes |
---|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. for 30 minutes at RT
|
Target
-
Function
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. -
Sequence similarities
Contains 3 SRCR domains. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 921 Human
- Omim: 153340 Human
- SwissProt: P06127 Human
- Unigene: 58685 Human
-
Alternative names
- CD 5 antibody
- CD5 antibody
- CD5 antigen (p56 62) antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab213004 has not yet been referenced specifically in any publications.