Overview

  • Product name
    Anti-CD62P antibody [Psel.KO.2.7]
    See all CD62P primary antibodies
  • Description
    Mouse monoclonal [Psel.KO.2.7] to CD62P
  • Host species
    Mouse
  • Specificity
    This antibody recognizes the CD62P cell surface antigen
  • Tested applications
    Suitable for: IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Human
  • Immunogen

    CD62P transfected 300.19 cells (Human)

  • Positive control
    • Thrombin activated human platelets
  • General notes
    Shelf Life - 18 months, product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab54427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: 1/25 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
    • Tissue specificity
      Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
    • Involvement in disease
      Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    • Sequence similarities
      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 9 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Images

    • ab54427, at a 1/25 dilution, staining thrombin activated human platelets

    References

    This product has been referenced in:
    • Li XH  et al. Effect of Platelet-derived P-selectin on Neutrophil Recruitment in a Mouse Model of Sepsis-induced Acute Kidney Injury. Chin Med J (Engl) 130:1694-1699 (2017). Mouse . Read more (PubMed: 28685720) »
    • Holloway PM  et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). Read more (PubMed: 27401964) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for your reply.

    I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    To be able to help you further in this matter, would you be able to send me a copy of the protocol that you have been using, along with any modifications that you have tried, to see if there is anything I can suggest.

    I look forward to your reply and helping resolve this matter.

    Read More

    Answer

    Thank you for your reply.

    I have sent the quote for the products requested to the email address we have on file for **********.

    When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

    If there is anything else I can help you with, please let me know.

    Read More

    Question
    Answer

    Thank you for your reply.

    Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

    Thank you for your patience, and I look forward to your reply.

    Read More

    Question
    Answer

    Yes, you can use ab6947 with ab54427. The antibody that recognizes IgG will recognize all IgG subtypes, whereas the IgG1, will only recognize IgG. Therefore, if you are not intending to use any other mouse primary antibodies at the same time then the IgG antibody will work well, if however you would like to use other mouse antibodies, then I would recommend the IgG1.

    Please let me know if there is anything else I can help you with, and if you can provide me the address, I can generate a quote.

    Read More

    Answer

    Thank you for your inquiry. I am happy to confirm that we offer an antibody against P selectin (CD62P) that is tested and guaranteed for horse samples (the prize can be found on the top of the datasheet): ab54427 (Anti-CD62P antibody [Psel.KO.2.7]) Click here (or use the following: https://www.abcam.com/index.html?datasheet=54427). Unfortunately, we do not have any antibodies against CD45, CD11b or ETAR (endothelin type A receptor in horse available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html ETAR from horse shows a 96% homology with human and you might be eligible for our testing discount program. Please let me know what application are planned with this antibody. I hope this information is helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up