Anti-CD64 antibody [10.1] (ab119843)
Key features and details
- Mouse monoclonal [10.1] to CD64
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD64 antibody [10.1]
See all CD64 primary antibodies -
Description
Mouse monoclonal [10.1] to CD64 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD64. Rheumatoid synovial fluid cells and fibronectin purified Human monocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab119843 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
10.1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- FITC Anti-CD64 antibody [10.1] (ab119828)
- Anti-CD64 antibody [10.1] - Low endotoxin, Azide free (ab185738)
- PE/Cy5® Anti-CD64 antibody [10.1] (ab192338)
- Alexa Fluor® 647 Anti-CD64 antibody [10.1], prediluted (ab192343)
- PE/Cy7® Anti-CD64 antibody [10.1] (ab201318)
- PE Anti-CD64 antibody [10.1] (ab23890)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
Tissue specificity
Monocyte/macrophage specific. -
Sequence similarities
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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Database links
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
Alternative names
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
Images
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Surface staining of CD64 in human peripheral blood with anti-CD64 (10.1) purified , GAM-APC.
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Flow Cytometry analysis of human peripheral whole blood labeling CD64 positive monocytes with ab119843 at 4 μg/mL (red-filled).
Negative control: CD64 negative lymphocytes (black-dashed).
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Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab119843 has been referenced in 9 publications.
- Heylmann D et al. Comparison of DNA repair and radiosensitivity of different blood cell populations. Sci Rep 11:2478 (2021). PubMed: 33510180
- Zhang L et al. C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/ß-catenin and ERK signaling in streptozocin-induced diabetic nephropathy. FASEB J 33:6551-6563 (2019). PubMed: 30794428
- Bajpai G et al. The human heart contains distinct macrophage subsets with divergent origins and functions. Nat Med 24:1234-1245 (2018). PubMed: 29892064
- McGuinness D et al. A molecular signature for delayed graft function. Aging Cell 17:e12825 (2018). PubMed: 30094915
- Kozicky LK et al. IVIg and LPS Co-stimulation Induces IL-10 Production by Human Monocytes, Which Is Compromised by an Fc?RIIA Disease-Associated Gene Variant. Front Immunol 9:2676 (2018). PubMed: 30515163
- Iruarrizaga-Lejarreta M et al. Role of Aramchol in steatohepatitis and fibrosis in mice. Hepatol Commun 1:911-927 (2017). IHC-P . PubMed: 29159325
- Sánchez-Torres C et al. CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol 13:1571-81 (2001). PubMed: 11717198
- Jayaram Y et al. The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma. Clin Exp Immunol 75:414-20 (1989). PubMed: 2495203
- Dougherty GJ et al. The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur J Immunol 17:1453-9 (1987). PubMed: 3500057