• Product name
    Anti-CD8 alpha antibody [53-6.7]
    See all CD8 alpha primary antibodies
  • Description
    Rat monoclonal [53-6.7] to CD8 alpha
  • Host species
  • Specificity
    ab25478 recognises a chain of the murine CD8 heterodimer.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
    Affinity purified
  • Primary antibody notes
    This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • Clonality
  • Clone number
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab25478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration. Acetone fixed.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
(In vivo and in vitro depletion of CD8a+ cells): Use at an assay dependent dilution.
Fractionation of CD8a+ T cells: Use at an assay dependent dilution.


  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all


  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD8 alpha with ab25478 at 1 μg/106 cells, and Rat Anti-Mouse CD4-UNLB, followed by Mouse Anti-Rat IgG2a-AF647 and Mouse Anti-Rat IgG2b-AF488.

  • ab25478 staining CD8 alpha in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®594 was used as secondary antibody at a 1/250 dilution.

    See Abreview


This product has been referenced in:
  • Yoo B  et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). Read more (PubMed: 30886310) »
  • Guan C  et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). Read more (PubMed: 29456788) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


I can confirm that these two antibodies ab25478 and ab25475 are tested externally in functional studies.

There are publications where these clones have been tested in this application. I have attached thse for your review and hope this will be helpful.

The protocol referenced for CD4 antibody [GK1.5] can be found in the following book. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.

I hope this information will be helpful. Please do not hesitae to let me know if you have any questions or if I may assist you further.

Read More


Thank you for contacting us. We have many other products that works well on mouse tissues and are fully tested. The catalogue numbers are Un conjugated anti CD8 antibodies ab22378 ab25478 ab3081 ab108292 Conjugated anti CD8 antibodies ab25294 ab25676 ab60077 ab25499 ab25498 ab39853 These antibodies will work on mouse section in the applications as recommended on datasheet. Please check the datasheets and buy the antibody as per your requirements. All antibodies are equally good and are fully guaranteed under Abpromise to work with mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Immunocytochemistry/ Immunofluorescence
Mouse Cell (bone marrow)
bone marrow
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 19 2011

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (spleen)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 30 2010

For licensing inquiries, please contact partnerships@abcam.com

Sign up