Product nameAnti-CD86 antibody
See all CD86 primary antibodies
DescriptionRabbit polyclonal to CD86
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD86.
- Recombinant Human CD86 protein (ab114228) can be used as a positive control in WB. This antibody gave a positive signal in Human spleen tissue lysate as well as the following whole cell lysates: Raji; Ramos; Jurkat; MOLT4; HeLa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
All lanes : Anti-CD86 antibody (ab79683) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 150 kDa, 35 kDa (possible cleavage fragment), 41 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 35 kDa represents the mature form of CD86 due to the presence of a 23 amino acid signal peptide. The band observed at 41 kDa represents the pro-form of CD86 as it contains a number of potential glycosylation sites (SwissProt).
ab79683 has not yet been referenced specifically in any publications.