Anti-CDK1 (phospho T161) antibody (ab47329)
Key features and details
- Rabbit polyclonal to CDK1 (phospho T161)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-CDK1 (phospho T161) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho T161) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CDK1 aa 100-200 (phospho T161).
Database link: P06493 -
Positive control
- WB: COLO, K362 cell extract. IHC-P: Human breast carcinoma tissue.
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General notes
This antibody detects CDC2 only when phosphorylated at Threonine 161.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Primary antibody notes
This antibody detects CDC2 only when phosphorylated at Threonine 161. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
Tissue specificity
Isoform 2 is found in breast cancer tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
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Form
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
Alternative names
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
Images
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All lanes : Anti-CDK1 (phospho T161) antibody (ab47329) at 1/500 dilution
Lane 1 : COLO cell extract preincubated with immunogenic phospho-peptide. with phosphopeptide at 1 µg/ml
Lane 2 : COLO cell extract, untreated.
Lane 3 : K362 cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa -
ab47329 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide using Alkaline phosphatase Goat anti rabbit IgG (H+L) secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab47329 has been referenced in 6 publications.
- Davies A et al. An androgen receptor switch underlies lineage infidelity in treatment-resistant prostate cancer. Nat Cell Biol 23:1023-1034 (2021). PubMed: 34489572
- Liu L et al. Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade. Dev Cell 52:196-209.e9 (2020). PubMed: 31866205
- Roseweir AK et al. Phosphorylation of androgen receptors at serine 515 is a potential prognostic marker for triple negative breast cancer. Oncotarget 8:37172-37185 (2017). IHC-P ; Human . PubMed: 28415597
- Wang J et al. Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9:1266-1272 (2015). IHC ; Mouse . PubMed: 25663895
- Willder JM et al. Androgen receptor phosphorylation at serine 515 by Cdk1 predicts biochemical relapse in prostate cancer patients. Br J Cancer 108:139-48 (2013). IHC . PubMed: 23321516
- Shirazi Fard S et al. Heterogenic final cell cycle by chicken retinal Lim1 horizontal progenitor cells leads to heteroploid cells with a remaining replicated genome. PLoS One 8:e59133 (2013). IF . PubMed: 23527113