Product nameAnti-CDKAL1 antibody
See all CDKAL1 primary antibodies
DescriptionRabbit polyclonal to CDKAL1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- This antibody gave a positive signal in the following whole cell lysates: HepG2, Jurkat, Raji and SK N BE. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PANC-1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCatalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
Tissue specificityExpressed in pancreatic islets.
Involvement in diseaseGenetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similaritiesBelongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain.
Cellular localizationEndoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
- Information by UniProt
- CDK5 regulatory subunit associated protein 1 like 1 antibody
- CDK5 regulatory subunit-associated protein 1-like 1 antibody
- CDKAL_HUMAN antibody
All lanes : Anti-CDKAL1 antibody (ab74020) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 8 minutes
ICC/IF image of ab74020 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74020 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74020 staining CDKAL1 in mouse pancreatic tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval and blocking (5 minutes/peroxidase block and 10 minutes/protein block) for 45 minutes at 200C. The primary antibody was diluted 1/250 and incubated with the sample for 45 minutes at 200C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
ab74020 staining CDKAL1 in human pancreatic tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval and blocking (5 minutes/peroxidase block and 10 minutes/protein block) for 15 minutes at 200C. The primary antibody was diluted 1/250 and incubated with the sample for 45 minutes at 200C. A HRP polymer conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
This product has been referenced in:
- Brambillasca S et al. CDK5 Regulatory Subunit-associated Protein 1-Like 1 (CDKAL1) Is a Tail-anchored Protein in the Endoplasmic Reticulum (ER) of Insulinoma Cells. J Biol Chem 287:41808-19 (2012). WB . Read more (PubMed: 23048041) »