Anti-CENPI antibody (ab118796)
Key features and details
- Rabbit polyclonal to CENPI
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CENPI antibody
See all CENPI primary antibodies -
Description
Rabbit polyclonal to CENPI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CENPI aa 50-150.
Database link: Q92674 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 87 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 87 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 2491 Human
- Omim: 300065 Human
- SwissProt: Q92674 Human
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Alternative names
- CENP I antibody
- centromere protein I antibody
- Follicle simulating hormone primary response protein antibody
see all
Images
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All lanes : Anti-CENPI antibody (ab118796) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 87 kDa
Exposure time: 30 seconds -
Detection of CENPI in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118796 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab118796 has been referenced in 4 publications.
- Wang J et al. Centromere Protein I (CENP-I) Is Upregulated in Gastric Cancer, Predicts Poor Prognosis, and Promotes Tumor Cell Proliferation and Migration. Technol Cancer Res Treat 20:15330338211045510 (2021). PubMed: 34617858
- Engqvist H et al. Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry. Front Oncol 10:162 (2020). PubMed: 32133296
- Kyriacou E & Heun P High-resolution mapping of centromeric protein association using APEX-chromatin fibers. Epigenetics Chromatin 11:68 (2018). PubMed: 30445992
- Thangavelu PU et al. Overexpression of the E2F target geneCENPIpromotes chromosome instability and predicts poor prognosis in estrogen receptor-positive breast cancer. Oncotarget 8:62167-62182 (2017). PubMed: 28977935