Anti-CHD8 antibody (ab84527)
Key features and details
- Rabbit polyclonal to CHD8
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CHD8 antibody
See all CHD8 primary antibodies -
Description
Rabbit polyclonal to CHD8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Chimpanzee, Monkey, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide within Human CHD8 aa 2250-2350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_065971.2 (GeneID 57680).
Database link: Q9HCK8 -
Positive control
- IHC-P: Human breast carcinoma and prostate carcinoma tissue.
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General notes
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355
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IP |
Use at an assay dependent concentration. PubMed: 23285124
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Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 291 kDa. PubMed: 24705355 |
IP
Use at an assay dependent concentration. PubMed: 23285124 |
Target
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Function
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites. - Information by UniProt
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Database links
- Entrez Gene: 57680 Human
- Entrez Gene: 67772 Mouse
- Omim: 610528 Human
- SwissProt: Q9HCK8 Human
- SwissProt: Q09XV5 Mouse
- Unigene: 530698 Human
- Unigene: 289934 Mouse
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Alternative names
- ATP dependent helicase CHD8 antibody
- ATP-dependent helicase CHD8 antibody
- Axis duplication inhibitor antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab84527 has been referenced in 7 publications.
- Kweon H et al. Excitatory neuronal CHD8 in the regulation of neocortical development and sensory-motor behaviors. Cell Rep 34:108780 (2021). PubMed: 33626347
- Jiménez JA et al. Chd8 haploinsufficiency impairs early brain development and protein homeostasis later in life. Mol Autism 11:74 (2020). PubMed: 33023670
- Zhao C et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell 45:753-768.e8 (2018). PubMed: 29920279
- Akhade VS et al. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res 44:387-401 (2016). PubMed: 26446991
- Tahara T et al. Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. Gastroenterology 146:530-538.e5 (2014). IHC-P ; Human . PubMed: 24211491
- Schulz Y et al. CHARGE and Kabuki syndromes: a phenotypic and molecular link. Hum Mol Genet N/A:N/A (2014). WB ; Human . PubMed: 24705355
- Batsukh T et al. Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex. PLoS One 7:e52640 (2012). IP ; Human . PubMed: 23285124