Overview

  • Product name
  • Description
    Rabbit polyclonal to CITED2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human CITED2 aa 4-191. The exact sequence is proprietary.
    Database link: Q99967

  • Positive control
    • 293T, HeLa, HepG2 whole cell lysates, Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab184145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP.
  • Sequence similarities
    Belongs to the CITED family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASD8 antibody
    • Cbp/p300 interacting transactivator 2 antibody
    • Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2 antibody
    • Cbp/p300-interacting transactivator 2 antibody
    • CITE2_HUMAN antibody
    • CITED 2 antibody
    • CITED2 antibody
    • melanocyte-specific gene 1-related gene 1 antibody
    • MRG 1 antibody
    • MRG-1 antibody
    • MRG1 antibody
    • MSG related protein 1 antibody
    • MSG-related protein 1 antibody
    • MSG1-related gene 1 antibody
    • P35srj antibody
    • VSD2 antibody
    see all

Images

  • Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde at room temperature for 15 min labeling CITED2 with ab184145 at 1/1000 (green) and Hoechst 33342 (blue).

  • All lanes : Anti-CITED2 antibody (ab184145) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Hela whole cell lysate
    Lane 3 : HepG2 whole cell lysate


    Lysates/proteins at 30 µg per lane.

    Predicted band size: 28 kDa



    12% SDS PAGE

References

ab184145 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Stem Cells)
Permeabilization
Yes - 0.2% TritonX
Specification
Embryonic Stem Cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 10 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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