Anti-CKAP1/TBCB antibody (ab96101)
Key features and details
- Rabbit polyclonal to CKAP1/TBCB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CKAP1/TBCB antibody
See all CKAP1/TBCB primary antibodies -
Description
Rabbit polyclonal to CKAP1/TBCB -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human CKAP1/TBCB aa 1-213.
Database link: NP_001272 -
Positive control
- WB: A431, H1299, HeLa and NIH/3T3 whle cell lysates. IHC-P: H1299 xenograft ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth. -
Tissue specificity
Found in most tissues. -
Sequence similarities
Belongs to the TBCB family.
Contains 1 CAP-Gly domain. -
Post-translational
modificationsPhosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone. - Information by UniProt
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Database links
- Entrez Gene: 1155 Human
- Entrez Gene: 66411 Mouse
- Entrez Gene: 292777 Rat
- Omim: 601303 Human
- SwissProt: Q99426 Human
- SwissProt: Q9D1E6 Mouse
- Unigene: 31053 Human
- Unigene: 27947 Mouse
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Alternative names
- CG22 antibody
- CKAPI antibody
- Cytoskeleton associated protein 1 antibody
see all
Images
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All lanes : Anti-CKAP1/TBCB antibody (ab96101) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Methanol-fixed A549 (human lung carcinoma cell line) cells labeling CKAP1/TBCB using ab96101 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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ab96101, at a 1/500 dilution, staining CKAP1/TBCB in paraffin embedded H1299 xenograft by Immunohistochemical analysis.
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Anti-CKAP1/TBCB antibody (ab96101) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab96101 has been referenced in 1 publication.
- Gong J et al. Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury. Biomed Rep 6:525-531 (2017). PubMed: 28515911