Anti-CKAP4 antibody (ab152154)
Key features and details
- Rabbit polyclonal to CKAP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CKAP4 antibody
See all CKAP4 primary antibodies -
Description
Rabbit polyclonal to CKAP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 245-573 of CKAP4 (UniProt ID: Q07065).
-
Positive control
- A549, HeLa, HepG2, HCT116 and MDA-MB-231 whole cell lysate; HeLa cells; CL1-5 xenograft tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000 - 1/20000. Predicted molecular weight: 66 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/5000 - 1/20000. Predicted molecular weight: 66 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
-
Post-translational
modificationsReversibly palmitoylated. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. - Information by UniProt
-
Database links
- Entrez Gene: 10970 Human
- SwissProt: Q07065 Human
- Unigene: 74368 Human
-
Alternative names
- 63 kDa membrane protein antibody
- CKAP 4 antibody
- Ckap4 antibody
see all
Images
-
Anti-CKAP4 antibody (ab152154) at 1/10000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 66 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells, labeling CKAP4 using ab152154 at 1/200 dilution. Lower image costained with Hoerchst 33342.
-
Immunohistochemical analysis of paraffin embedded CL1-5 xenograft tissue, labeling CKAP4 using ab152154 at 1/500 dilution.
-
Anti-CKAP4 antibody (ab152154) at 1/2000 dilution + MDA-MB-231 whole cell lysate at 30 µg with 5% non-fat milk in TBST
Predicted band size: 66 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab152154 has been referenced in 2 publications.
- Vaklavas C et al. Hallmarks and Determinants of Oncogenic Translation Revealed by Ribosome Profiling in Models of Breast Cancer. Transl Oncol 13:452-470 (2020). PubMed: 31911279
- Vaklavas C et al. Translational control of the undifferentiated phenotype in ER-positive breast tumor cells: Cytoplasmic localization of ERa and impact of IRES inhibition. Oncol Rep 39:2482-2498 (2018). PubMed: 29620220