Overview

  • Product name
    Anti-CKS1 antibody
  • Description
    Rabbit polyclonal to CKS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the N terminus of human CKS1.

  • Positive control
    • Extracts from Jurkat cells, treated with serum (20%, 15mins). HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/20000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Detects a band of <19 kDa (predicted molecular weight: 10 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
    • Sequence similarities
      Belongs to the CKS family.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC2 associated protein CKS1 antibody
      • CDC28 protein kinase 1 antibody
      • CDC28 protein kinase 1B antibody
      • CDC28 protein kinase regulatory subunit 1B antibody
      • Cell division control protein CKS1 antibody
      • CKS 1 antibody
      • CKS-1 antibody
      • CKS1 antibody
      • CKS1_HUMAN antibody
      • Cks1b antibody
      • Ckshs1 antibody
      • Cyclin dependent kinases regulatory subunit 1 antibody
      • Cyclin-dependent kinases regulatory subunit 1 antibody
      • NB4 apoptosis/differentiation related protein antibody
      • PNAS 143 antibody
      • PNAS 16 antibody
      • PNAS 18 antibody
      see all

    Images

    • All lanes : Anti-CKS1 antibody (ab72639) at 1/500 dilution

      Lane 1 : Extracts from Jurkat cells, treated
      with serum (20%, 15mins)
      Lane 2 : Extracts from Jurkat cells, treated
      with serum (20%, 15mins) with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 10 kDa
      Observed band size: <19 kDa
      why is the actual band size different from the predicted?

    • ab72639, at 1/500 dilution, staining CKS1 in HeLa cells by Immunofluorescence in the absence or presence of the immunising peptide.

    References

    ab72639 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply.
    Unfortunately, as we carry over 90,000 products, it simply isn't feasible for us to keep small sample sizes of any of our products.
    I would however be able to offer you a testing discount if you would be interested. This offer means that if you purchase one of the monoclonal antibodies against CKS2 as normal, test the antibody with your mouse samples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of the antibody which was tested off any product). More information on this offer can be found from the following link:
    www.abcam.com/collaborationdiscount
    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.
    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of the antibody to be tested.
    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    Thank you for contacting us and your interest in our products.
    I have had a look into your request to see if any of the antibodies we have directed against CKS1 and CKS2 are likely to show specificity towards its target (i.e. not cross react with CKS2 and CKS1 respectively).
    Unfortunately, none of our antibodies against these targets have been tested for their specificity and the homology between the two proteins is very high. From the sequence alignment I have attached, the homology is 81% and the only region of great difference is from residues 12-17 and 25-30. In order to distinguish the two you would therefore need an antibody raised against a peptide taken from these regions and preferably a monoclonal antibody to ensure the consistency of the specificity.
    Unfortunately, we do not have any antibodies currently in our catalogue which matches these criteria. The majority of our antibodies against these targets are rabbit polyclonals raised against large regions of the protein. Even the monoclonal antibodies we have would not be suitable as they have been raised against the full length protein and whilst they may be able to differentiate between the proteins, it would be more likely that they would not be able to.
    I am sorry that I could not be of more help in this instance. If you have any further questions, please do not hesitate to contact us again.

    Read More

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