Anti-CLK1 antibody (ab74044)
Key features and details
- Rabbit polyclonal to CLK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CLK1 antibody
See all CLK1 primary antibodies -
Description
Rabbit polyclonal to CLK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
A synthesized peptide derived from the internal region of human CLK1.
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Positive control
- HUVEC cells and extracts from 293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 57 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 57 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylates on all three types of residues. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1195 Human
- Entrez Gene: 12747 Mouse
- Omim: 601951 Human
- SwissProt: P49759 Human
- SwissProt: P22518 Mouse
- Unigene: 433732 Human
- Unigene: 1761 Mouse
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Alternative names
- CDC like kinase 1 antibody
- CDC-like kinase 1 antibody
- CDC28/CDC2 like kinase antibody
see all
Images
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All lanes : Anti-CLK1 antibody (ab74044) at 1/500 dilution
Lane 1 : Extracts from 293 cells
Lane 2 : Extracts from 293 cells with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 30 kDa, 34 kDa, 36 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of CLK1 in HUVEC cells, using ab74044 at a 1/500 dilution.
Left image: untreated.
Right image: treated with immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab74044 has been referenced in 5 publications.
- Peciuliene I et al. Short-Term Hypoxia in Cells Induces Expression of Genes Which Are Enhanced in Stressed Cells. Genes (Basel) 13:N/A (2022). PubMed: 36140764
- Wang C et al. SRPK1 acetylation modulates alternative splicing to regulate cisplatin resistance in breast cancer cells. Commun Biol 3:268 (2020). PubMed: 32461560
- Zhou Q et al. Targeting CLK3 inhibits the progression of cholangiocarcinoma by reprogramming nucleotide metabolism. J Exp Med 217:N/A (2020). PubMed: 32453420
- Fant X et al. cdc-like/dual-specificity tyrosine phosphorylation-regulated kinases inhibitor leucettine L41 induces mTOR-dependent autophagy: implication for Alzheimer's disease. Mol Pharmacol 85:441-50 (2014). PubMed: 24366666
- Ninomiya K et al. Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor. J Cell Biol 195:27-40 (2011). WB . PubMed: 21949414