Recombinant Anti-COX5A antibody [EPR14208(B)] (ab180129)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14208(B)] to COX5A
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-COX5A antibody [EPR14208(B)]
See all COX5A primary antibodies -
Description
Rabbit monoclonal [EPR14208(B)] to COX5A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cell lysate; human heart, mouse heart and brain, rat heart and brain tissue lysates. IHC-P: Human colon carcinoma, mouse kidney and rat stomach tissue. IP: Mouse heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14208(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. |
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WB |
1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).
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IP |
1/80.
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Notes |
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IHC-P
1/3000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. |
WB
1/5000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa). |
IP
1/80. |
Target
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Function
This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
Sequence similarities
Belongs to the cytochrome c oxidase subunit 5A family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 9377 Human
- Entrez Gene: 12858 Mouse
- Entrez Gene: 252934 Rat
- Omim: 603773 Human
- SwissProt: P20674 Human
- SwissProt: P12787 Mouse
- SwissProt: P11240 Rat
- Unigene: 401903 Human
see all -
Alternative names
- COX antibody
- COX VA antibody
- COX5A antibody
see all
Images
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All lanes : Anti-COX5A antibody [EPR14208(B)] (ab180129) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in PMID: 28082314
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling COX5A with purified ab180129 at 1/3000 dilution (0.5 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Purified ab180129 at 1/80 dilution (2ug) immunoprecipitating COX5A in Mouse heart lysate.
Lane 1 (input): Mouse heart lysate (10µg)
Lane 2 (+): ab180129 + Mouse heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180129 in Mouse heart lysate.
Lane 4: Mouse heart lysate captured by ab180129 and detected by anti-Ubiquitin antibody (ab134953).
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 13 kDa
The ladder band pattern could be ubiquitylated Cox5a as validated by anti-Ubiquitin antibody (ab134953, Lane4) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling COX5A with purified ab180129 at 1/3000 dilution (0.5 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX5A with purified ab180129 at 1/3000 dilution (0.5 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab180129 has been referenced in 5 publications.
- Karki M et al. A cytoskeletal function for PBRM1 reading methylated microtubules. Sci Adv 7:N/A (2021). PubMed: 33811077
- Stokum JA et al. A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain. J Cereb Blood Flow Metab 41:2546-2560 (2021). PubMed: 33818185
- Chong SJF et al. Serine-70 phosphorylated Bcl-2 prevents oxidative stress-induced DNA damage by modulating the mitochondrial redox metabolism. Nucleic Acids Res 48:12727-12745 (2020). PubMed: 33245769
- Huang Y et al. The Dual Role of AQP4 in Cytotoxic and Vasogenic Edema Following Spinal Cord Contusion and Its Possible Association With Energy Metabolism via COX5A. Front Neurosci 13:584 (2019). PubMed: 31258460
- Das S et al. miR-181c Regulates the Mitochondrial Genome, Bioenergetics, and Propensity for Heart Failure In Vivo. PLoS One 9:e96820 (2014). WB ; Rat . PubMed: 24810628