Overview

  • Product name
    Anti-CPSF6 antibody [EPR12898]
    See all CPSF6 primary antibodies
  • Description
    Rabbit monoclonal [EPR12898] to CPSF6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human CPSF6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q16630

  • Positive control
    • HeLa, 293T, Jurkat and K562 cell lysates; Human breast and Human colon carcinoma tissues; HeLa cells; Jurkat cells; K562 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12898
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 59 kDa.

Target

  • Function
    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
  • Sequence similarities
    Belongs to the RRM CPSF6/7 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFIM antibody
    • CFIm68 antibody
    • cleavage and polyadenylation specific factor 6, 68kDa antibody
    • Cleavage and polyadenylation specificity factor 68 kDa subunit antibody
    • Cleavage and polyadenylation specificity factor subunit 6 antibody
    • cleavage factor Im complex 68 kDa subunit antibody
    • CPSF 68 kDa subunit antibody
    • Cpsf6 antibody
    • CPSF6_HUMAN antibody
    • HPBRII 4 antibody
    • HPBRII 7 antibody
    • Pre mRNA cleavage factor Im 68 kDa subunit antibody
    • pre-mRNA cleavage factor I, 68kD subunit antibody
    • pre-mRNA cleavage factor Im (68kD) antibody
    • Pre-mRNA cleavage factor Im 68 kDa subunit antibody
    • Protein HPBRII 4/7 antibody
    • Protein HPBRII-4/7 antibody
    see all

Images

  • All lanes : Anti-CPSF6 antibody [EPR12898] (ab175237) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa

  • Immunofluorescent analysis of HeLa cells labeling CPSF6 with ab175237 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human breast tissue labeling CPSF6 with ab175237 at 1/100.

  • Immunoprecipitation of CPSF6 from HeLa cell lysate using ab175237 at 1/10 labeled with ab175237 at 1/10.

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling CPSF6 with ab175237 at 1/100.

  • Flow Cytometrical analysis of permeabilized Jurakt cells labeling CPSF6 with ab175237 antibody at 1/10 (red) or a rabbit IgG negative (green).

References

This product has been referenced in:
  • Chang JW  et al. An integrative model for alternative polyadenylation, IntMAP, delineates mTOR-modulated endoplasmic reticulum stress response. Nucleic Acids Res 46:5996-6008 (2018). Read more (PubMed: 29733382) »
  • Binothman N  et al. CPSF6 is a Clinically Relevant Breast Cancer Vulnerability Target: Role of CPSF6 in Breast Cancer. EBioMedicine 21:65-78 (2017). IHC-P ; Human . Read more (PubMed: 28673861) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Quail Cell lysate - nuclear (QT6 cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
5 µg
Specification
QT6 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2015

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