Product nameAnti-CPSF6 antibody [EPR12898]
See all CPSF6 primary antibodies
DescriptionRabbit monoclonal [EPR12898] to CPSF6
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis
Synthetic peptide within Human CPSF6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16630
- HeLa, 293T, Jurkat and K562 cell lysates; Human breast and Human colon carcinoma tissues; HeLa cells; Jurkat cells; K562 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 59 kDa.|
FunctionComponent of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
Sequence similaritiesBelongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles.
- Information by UniProt
- CFIM antibody
- CFIm68 antibody
- cleavage and polyadenylation specific factor 6, 68kDa antibody
All lanes : Anti-CPSF6 antibody [EPR12898] (ab175237) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa
Immunofluorescent analysis of HeLa cells labeling CPSF6 with ab175237 at 1/100.
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling CPSF6 with ab175237 at 1/100.
Immunoprecipitation of CPSF6 from HeLa cell lysate using ab175237 at 1/10 labeled with ab175237 at 1/10.
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling CPSF6 with ab175237 at 1/100.
Flow Cytometrical analysis of permeabilized Jurakt cells labeling CPSF6 with ab175237 antibody at 1/10 (red) or a rabbit IgG negative (green).
This product has been referenced in:
- Chang JW et al. An integrative model for alternative polyadenylation, IntMAP, delineates mTOR-modulated endoplasmic reticulum stress response. Nucleic Acids Res 46:5996-6008 (2018). Read more (PubMed: 29733382) »
- Binothman N et al. CPSF6 is a Clinically Relevant Breast Cancer Vulnerability Target: Role of CPSF6 in Breast Cancer. EBioMedicine 21:65-78 (2017). IHC-P ; Human . Read more (PubMed: 28673861) »