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Rabbit polyclonal to CSTF2T
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Gorilla, Soybean, Orangutan, Elephant
Synthetic peptide corresponding to a region between residues 566 and 616 of Human CSTF2T (NP_056050.1).
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).
Use at 2-5 µg/mg of lysate.
May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs.
Contains 1 RRM (RNA recognition motif) domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
CF 1 64 kDa subunit tau antibody
CF-1 64 kDa subunit tau variant antibody
CF1 64 kDa subunit, tau variant antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSTF2T antibody (ab80275)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CSTF2T with ab80275 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-CSTF2T antibody (ab80275)
All lanes :
Anti-CSTF2T antibody (ab80275) at 0.4 µg/ml
Lane 1 :
Whole cell lysate from HeLa cells at 50 µg
Lane 2 :
Whole cell lysate from HeLa cells at 15 µg
Lane 3 :
Whole cell lysate from HeLa cells at 5 µg
Predicted band size:
Observed band size:
70 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-CSTF2T antibody (ab80275)
Detection of CSTF2T in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80275 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"