PE Anti-CTLA4 antibody [UC10-4F10-11] (ab210383)
Key features and details
- PE Armenian hamster monoclonal [UC10-4F10-11] to CTLA4
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Overview
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Product name
PE Anti-CTLA4 antibody [UC10-4F10-11]
See all CTLA4 primary antibodies -
Description
PE Armenian hamster monoclonal [UC10-4F10-11] to CTLA4 -
Host species
Armenian hamster -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse -
Immunogen
Fusion protein corresponding to Mouse CTLA4. Mouse CTLA-4 IgG2a Fusion
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Positive control
- Flow Cyt (Intra): C57Bl/6 splenocytes
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General notes
ab210383 was conjugated under optimal conditions, with unreacted dye removed from the preparation.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Monobasic dihydrogen sodium phosphate, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
ab210383 was purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
UC10-4F10-11 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. -
Tissue specificity
Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. -
Involvement in disease
Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Note=Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism.
Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12) [MIM:601388]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylation is important for dimerization.
Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. -
Cellular localization
Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and. - Information by UniProt
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Database links
- Entrez Gene: 100505288 Mouse
- Entrez Gene: 12477 Mouse
- SwissProt: P09793 Mouse
- Unigene: 390 Mouse
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Alternative names
- ALPS5 antibody
- CD 152 antibody
- CD antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab210383 has not yet been referenced specifically in any publications.