Anti-CXCL14 antibody (ab46010)
Key features and details
- Rabbit polyclonal to CXCL14
- Suitable for: Sandwich ELISA, ICC/IF, IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CXCL14 antibody
See all CXCL14 primary antibodies -
Description
Rabbit polyclonal to CXCL14 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, ICC/IF, IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant hBRAK (human BRAK).
-
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Lyophilized:Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect CXCL14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. |
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducing conditions. |
|
ELISA |
Use at an assay dependent concentration.
Detection limit 0.2 - 0.4 ng/well. |
Notes |
---|
Sandwich ELISA
Use at an assay dependent concentration. To detect CXCL14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducing conditions. |
ELISA
Use at an assay dependent concentration. Detection limit 0.2 - 0.4 ng/well. |
Target
-
Function
Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 9547 Human
- Omim: 604186 Human
- SwissProt: O95715 Human
- Unigene: 483444 Human
-
Alternative names
- 1110031L23Rik antibody
- 1200006I23Rik antibody
- AI414372 antibody
see all
Protocols
Datasheets and documents
-
Datasheet download
References (10)
ab46010 has been referenced in 10 publications.
- Xu K et al. Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis. Oncogenesis 10:66 (2021). PubMed: 34611125
- Niu L et al. Two coupled mutations abolished the binding of CEBPB to the promoter of CXCL14 that displayed an antiviral effect on PRRSV by activating IFN signaling. FASEB J 34:11257-11271 (2020). PubMed: 32648265
- Sata Y et al. High expression of CXCL14 is a biomarker of lung adenocarcinoma with micropapillary pattern. Cancer Sci 111:2588-2597 (2020). PubMed: 32403160
- Ji X et al. CXCL14 and NOS1 expression in specimens from patients with stage I-IIIA nonsmall cell lung cancer after curative resection. Medicine (Baltimore) 97:e0101 (2018). IHC ; Human . PubMed: 29517684
- Wang Y et al. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest 128:5235-5250 (2018). PubMed: 30204129
- Alexiadis M et al. Transcriptomic analysis of stage 1 versus advanced adult granulosa cell tumors. Oncotarget 7:14207-19 (2016). IHC . PubMed: 26893359
- Riester M et al. Risk Prediction for Late-Stage Ovarian Cancer by Meta-analysis of 1525 Patient Samples. J Natl Cancer Inst 106:N/A (2014). IHC-P ; Human . PubMed: 24700803
- Wang W et al. Antitumor efficacy of C-X-C motif chemokine ligand 14 in hepatocellular carcinoma in vitro and in vivo. Cancer Sci 104:1523-31 (2013). PubMed: 24033560
- Leach RE et al. High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod 27:814-828 (2012). IHC-P ; Human . PubMed: 22215622
- Gu XL et al. Expression of CXCL14 and its anticancer role in breast cancer. Breast Cancer Res Treat 135:725-35 (2012). WB . PubMed: 22910931