• Product name
  • Description
    Rabbit polyclonal to CYP27B1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human CYP27B1 aa 482-508 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 1594

  • Positive control
    • WB: Mouse kidney tissue lysate. IHC-P: Human kidney tissue. Flow Cyt: 293 cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • Clonality
  • Isotype


Our Abpromise guarantee covers the use of ab95047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Function
    Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
  • Tissue specificity
  • Pathway
    Hormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in disease
    Defects in CYP27B1 are the cause of rickets vitamin D-dependent type 1A (VDDR1A) [MIM:264700]; also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1alpha(OH)ase antibody
    • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase antibody
    • 25-hydroxyvitamin D-1 alpha hydroxylase antibody
    • 25-OHD-1 alpha-hydroxylase antibody
    • Calcidiol 1-monooxygenase antibody
    • CP27B_HUMAN antibody
    • CP2B antibody
    • CYP1 antibody
    • CYP1ALPHA antibody
    • CYP27B antibody
    • Cyp27b1 antibody
    • Cytochrome p450 27B1 antibody
    • Cytochrome p450 27B13 antibody
    • Cytochrome P450 family 27 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIB polypeptide 1 antibody
    • Cytochrome P450C1 alpha antibody
    • Cytochrome P450VD1-alpha antibody
    • mitochondrial antibody
    • P450C1 alpha antibody
    • P450c1 antibody
    • P450C1-alpha antibody
    • P450VD1-alpha antibody
    • PDDR antibody
    • VD3 1A hydroxylase antibody
    • VDD1 antibody
    • VDDR antibody
    • VDDR I antibody
    • VDDRI antibody
    • VDR antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CYP27B1 with ab95047. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-CYP27B1 antibody (ab95047) at 1/1000 dilution + T47D cell line lysates at 20 µg

    Goat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

    Predicted band size: 57 kDa

  • Anti-CYP27B1 antibody (ab95047) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg

    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 57 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry analysis of 293 cells labelling CYP27B1 with ab95047 (green) compared to a negative control (blue). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the primary antibody (1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody, a FITC conjugated goat anti-rabbit IgG, was used at 1/200 dilution for 40 min at room temperature. Acquisition of >10,000 events was performed.


This product has been referenced in:
  • Bukuroshi P  et al. Strategies and limitations associated with in vitro characterization of vitamin D receptor activators. Biochem Pharmacol 155:547-561 (2018). Read more (PubMed: 30028992) »
  • Borges CC  et al. Vitamin D deficiency aggravates the liver metabolism and inflammation in ovariectomized mice. Biomed Pharmacother 107:878-888 (2018). Read more (PubMed: 30257400) »
See all 2 Publications for this product

Customer reviews and Q&As


Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:


I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up