Anti-Calnexin antibody (ab10286)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Calnexin antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin -
Host species
Rabbit -
Specificity
Recognizes ER membrane, mitochondria and cis-Golgi -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Dog Calnexin aa 1-500. Expressed in E. coli. N-terminal ER luminal domain.
Database link: P24643 -
Positive control
- WB: Human CEM T-lymphoblastoid leukemia cell lines. MCF cells. HeLa total cell lysate. HeLa membrane fraction. MDA-MB231 total cell lysate. MDA-MB231 membrane fraction.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 99% Rabbit Serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (14) |
1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.
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Notes |
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WB
1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Omim: 114217 Human
- SwissProt: P27824 Human
- Unigene: 567968 Human
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
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Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines. -
Anti-Calnexin antibody (ab10286) at 1/1000 dilution + MCF7 cells
Secondary
Goat anti rabbit at 1/10000 dilution
Predicted band size: 67 kDa -
All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : HeLa (Total cell lysate)
Lane 2 : HeLa (Membrane fraction)
Lane 3 : HeLa (Cytoplasmic fraction)
Lane 4 : MDA-MB231 (Total cell lysate)
Lane 5 : MDA-MB231 (Membrane fraction)
Lane 6 : MDA-MB231 (Cytoplasmic fraction)
Secondary
All lanes : HRP conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 5 minutes
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Anti-Calnexin antibody (ab10286) at 1/2000 dilution + HeLa whole cell lysate
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
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All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : CHO membrane fraction
Lane 2 : CHO cytosolic fraction
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution
Predicted band size: 67 kDa
Datasheets and documents
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Datasheet download
References (69)
ab10286 has been referenced in 69 publications.
- Xia KS et al. An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration. Bioact Mater 21:69-85 (2023). PubMed: 36017070
- Mohamud Yusuf A et al. Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles. Basic Res Cardiol 117:43 (2022). PubMed: 36038749
- Husein D et al. Identification of the C-terminal region in Amelogenesis Imperfecta causative protein WDR72 required for Golgi localization. Sci Rep 12:4640 (2022). PubMed: 35301423
- Pane K et al. Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study. Int J Mol Sci 23:N/A (2022). PubMed: 35409352
- Zou HY et al. Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice. J Clin Invest 132:N/A (2022). PubMed: 35426376