Anti-Calpain 2 antibody (ab39165)
Key features and details
- Rabbit polyclonal to Calpain 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Calpain 2 antibody
See all Calpain 2 primary antibodies -
Description
Rabbit polyclonal to Calpain 2 -
Host species
Rabbit -
Specificity
ab39165 binds to reduced and non-reduced calpain 2, but does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). It reacts with latent and active (amino-truncated) Calpain 2. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2. (Peptide available as ab41302.)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (6) |
1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection.
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IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 17192410
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection. |
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. PubMed: 17192410 |
Target
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Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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Database links
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Entrez Gene: 29154 Rat
- Omim: 114230 Human
- SwissProt: P17655 Human
- SwissProt: O08529 Mouse
- SwissProt: Q07009 Rat
- Unigene: 350899 Human
see all -
Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
see all
Images
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ab39165 was shown to react with CAPN2 in wild-type MDA-MB-231 (wpxld GFP) cells in Western blot with loss of signal observed in a CAPN2 knockout cell line. Wild-type MDA-MB-231 (wpxld GFP) and CAPN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab39165 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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All lanes : Anti-Calpain 2 antibody (ab39165) at 1/1000 dilution
Lane 1 : Calpain 2 control
Lane 2 : Cell lysates from human chondrosarcoma (treated with IL1 alpha) at 10 µg
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted? -
ab39165 (4µg/ml) staining CALPAIN2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Datasheets and documents
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SDS download
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Datasheet download
References (50)
ab39165 has been referenced in 50 publications.
- Lyu J & Ertbjerg P Sarcoplasmic and myofibril-bound calpains during storage of pork longissimus muscle: New insights on protein degradation. Food Chem 372:131347 (2022). PubMed: 34818749
- Yan J et al. Inherited Retinal Degeneration: PARP-Dependent Activation of Calpain Requires CNG Channel Activity. Biomolecules 12:N/A (2022). PubMed: 35327647
- Langlois B et al. LRP-1-dependent control of calpain expression and activity: A new mechanism regulating thyroid carcinoma cell adhesion. Front Oncol 12:981927 (2022). PubMed: 36052226
- Wang X et al. Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis. J Cell Mol Med 25:2238-2253 (2021). PubMed: 33439514
- Beryozkin A et al. A new mouse model for retinal degeneration due to Fam161a deficiency. Sci Rep 11:2030 (2021). PubMed: 33479377