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Our Abpromise guarantee covers the use of ab23703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).Can be blocked with Cannabinoid Receptor I peptide (ab50542).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/300. Detection with an ABC system is recommended.|
ab23703 staining Cannabinoid Receptor I in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
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