Anti-Carbonic anhydrase 2/CA2 antibody (ab191343)
Key features and details
- Rabbit polyclonal to Carbonic anhydrase 2/CA2
- Suitable for: ELISA, ICC/IF, IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic anhydrase 2/CA2 antibody
See all Carbonic anhydrase 2/CA2 primary antibodies -
Description
Rabbit polyclonal to Carbonic anhydrase 2/CA2 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, IHC-Fr, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length native protein (purified) corresponding to Human Carbonic anhydrase 2/CA2.
Database link: P00918 -
General notes
This product is the 100 µg version of ab6621.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 16586439
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WB |
1/2000 - 1/10000.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. PubMed: 16586439 |
WB
1/2000 - 1/10000. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. -
Involvement in disease
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 760 Human
- Entrez Gene: 12349 Mouse
- Omim: 611492 Human
- SwissProt: P00918 Human
- SwissProt: P00920 Mouse
- Unigene: 155097 Human
- Unigene: 1186 Mouse
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Alternative names
- CA 2 antibody
- CA II antibody
- CA-II antibody
see all
Images
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Anti-Carbonic anhydrase 2/CA2 antibody (ab191343) at 1/1000 dilution + Carbonic Anhydrase II at 0.05 µg
Secondary
Rabbit Peroxidase at 1/40000 dilution -
Formalin-fixed, paraffin-embedded human kidney tissue stained for Carbonic anhydrase 2/CA2 using ab191343 at 4 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded mouse eye tissue stained for Carbonic anhydrase 2/CA2 using ab191343 at 4 μg/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
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Datasheet download
References (17)
ab191343 has been referenced in 17 publications.
- Allgoewer K et al. New Proteomic Signatures to Distinguish Between Zika and Dengue Infections. Mol Cell Proteomics 20:100052 (2021). PubMed: 33582300
- Bernhardt A et al. High salt diet-induced proximal tubular phenotypic changes and sodium-glucose cotransporter-2 expression are coordinated by cold shock Y-box binding protein-1. FASEB J 35:e21912 (2021). PubMed: 34533842
- Arbez B et al. Human macrophages and osteoclasts resorb ß-tricalcium phosphate in vitro but not mouse macrophages. Micron 125:102730 (2019). PubMed: 31415983
- Hara ES et al. Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu. J Mater Chem B 6:6153-6161 (2018). PubMed: 32254824
- Bamba R et al. Evaluation of a Nerve Fusion Technique With Polyethylene Glycol in a Delayed Setting After Nerve Injury. J Hand Surg Am 43:82.e1-82.e7 (2018). IHC-P . PubMed: 28823535