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    cardiac-troponin-i-antibody-ab47003.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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Anti-Cardiac Troponin I antibody (ab47003)

  • Datasheet
  • SDS
Reviews (17)Q&A (5)References (110)

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Western blot - Anti-Cardiac Troponin I antibody (ab47003)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (ab47003)
  • Western blot - Anti-Cardiac Troponin I antibody (ab47003)
  • Western blot - Anti-Cardiac Troponin I antibody (ab47003)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (ab47003)

Key features and details

  • Rabbit polyclonal to Cardiac Troponin I
  • Suitable for: ELISA, WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Cardiac Troponin I antibody
    See all Cardiac Troponin I primary antibodies
  • Description

    Rabbit polyclonal to Cardiac Troponin I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Cardiac Troponin I aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab47002)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Cardiac Troponin I peptide (ab47002)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA (1)
Use at an assay dependent concentration.
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Cardiac Troponin I peptide (ab47002).
ICC/IF (5)
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 21909276

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (4)
1/100.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Cardiac Troponin I peptide (ab47002).
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 21909276

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/100.

Target

  • Function

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease

    Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities

    Belongs to the troponin I family.
  • Target information above from: UniProt accession P19429 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7137 Human
    • Entrez Gene: 21954 Mouse
    • Entrez Gene: 100049696 Pig
    • Entrez Gene: 29248 Rat
    • Omim: 191044 Human
    • SwissProt: P19429 Human
    • SwissProt: P48787 Mouse
    • SwissProt: P23693 Rat
    • Unigene: 709179 Human
    • Unigene: 27674 Mouse
    • Unigene: 64141 Rat
    see all
  • Alternative names

    • cardiac muscle antibody
    • Cardiac troponin I antibody
    • cardiomyopathy, dilated 2A (autosomal recessive) antibody
    • Cardiomyopathy, familial hypertrophic, 7, included antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnI antibody
    • Familial hypertrophic cardiomyopathy 7 antibody
    • MGC116817 antibody
    • RCM1 antibody
    • Tn1 antibody
    • Tni antibody
    • TNN I3 antibody
    • TNNC 1 antibody
    • TNNC1 antibody
    • TNNI3 antibody
    • TNNI3_HUMAN antibody
    • Troponin I antibody
    • Troponin I cardiac antibody
    • Troponin I cardiac muscle antibody
    • Troponin I cardiac muscle isoform antibody
    • Troponin I type 3 cardiac antibody
    • troponin I, cardiac 3 antibody
    • TroponinI antibody
    • Ttroponin I type 3 (cardiac) antibody
    see all

Images

  • Western blot - Anti-Cardiac Troponin I antibody (ab47003)
    Western blot - Anti-Cardiac Troponin I antibody (ab47003)
    All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (ab47003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (ab47003)
    IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47003, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot - Anti-Cardiac Troponin I antibody (ab47003)
    Western blot - Anti-Cardiac Troponin I antibody (ab47003)
    All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?
    Additional bands at: 160 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Western blot - Anti-Cardiac Troponin I antibody (ab47003)
    Western blot - Anti-Cardiac Troponin I antibody (ab47003)This image is courtesy of an Abreview submitted by Dr. Mario Torrado
    All lanes : Anti-Cardiac Troponin I antibody (ab47003) at 1/20000 dilution

    All lanes : Pig Heart whole cell lysate

    Lysates/proteins at 4 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Goat anti-rabbit polyclonal at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 29 kDa why is the actual band size different from the predicted?



    Blocking Step: 20% Serum for 3 hours at 24°C

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (ab47003)
    Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin I antibody (ab47003)This image is courtesy of an anonymous Abreview
    ab47003 staining Cardiac Troponin I in Human cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 20°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (110)

Publishing research using ab47003? Please let us know so that we can cite the reference in this datasheet.

ab47003 has been referenced in 110 publications.

  • Wei X  et al. Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats. Int J Mol Med 47:677-687 (2021). PubMed: 33416139
  • Ma Y  et al. SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice. Animal Model Exp Med 4:2-15 (2021). PubMed: 33738432
  • Zhang J  et al. Graphene Oxide and Reduced Graphene Oxide Exhibit Cardiotoxicity Through the Regulation of Lipid Peroxidation, Oxidative Stress, and Mitochondrial Dysfunction. Front Cell Dev Biol 9:616888 (2021). PubMed: 33816465
  • Lei Z  et al. miR-132/212 Impairs Cardiomyocytes Contractility in the Failing Heart by Suppressing SERCA2a. Front Cardiovasc Med 8:592362 (2021). PubMed: 33816571
  • Kofron CM  et al. A predictive in vitro risk assessment platform for pro-arrhythmic toxicity using human 3D cardiac microtissues. Sci Rep 11:10228 (2021). PubMed: 33986332
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 22 Abreviews or Q&A

ELISA abreview for Anti-Cardiac Troponin I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
Sample
Human Serum (immobilization on beads)
Specification
immobilization on beads
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Type
Sandwich (Capture)
Read More

Mr. Chang Su Jeon

Verified customer

Submitted Sep 19 2012

Immunocytochemistry abreview for Anti-Cardiac Troponin I antibody

Good
Abreviews
Abreviews
Application
Immunocytochemistry
Sample
Mouse Cell (Heart)
Permeabilization
No
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Ann Kurian

Verified customer

Submitted Apr 27 2021

Western blot abreview for Anti-Cardiac Troponin I antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

MRS. Talya Razin

Verified customer

Submitted Jun 11 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (post MI mouse heart)
Permeabilization
Yes - 3% triton
Specification
post MI mouse heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 29 2019

Immunocytochemistry abreview for Anti-Cardiac Troponin I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cultured Cells (Fetal Cardiomyocytes)
Specification
Fetal Cardiomyocytes
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde
Read More

Jennifer Dewing

Verified customer

Submitted Mar 24 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 19 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cardiac Troponin I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Mar 26 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cardiac Troponin I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Mar 22 2013

Question

Dear Tech Support Team,
Please read below and see the attached images (the troponin is green, cd31is pink) and advise to interpret the results.
Thanks in advance for your assistance.
Antibody code: ab47003,24590
Batch number:
General Information:
Antibody storage conditions (temperature/reconstitution etc) as stated in the date sheet. -20 in diluents
Description of the problem (high background, low signal, non-specific staining etc.)
non-specific staining and the staining itself is not like in the pictures in abcam webpage
Sample (Species/Tissue/Cell Type/Cell Line etc.) cells (cardiomyocytes and HUVEC on a scaffold
Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.) Methanol 10 min
Antigen retrieval (Enzymatic method, Heat mediated technique etc.) non needed
Permeabilization step tween 20 0.05% in PBS
Blocking conditions (Buffer/time period, Blocking agent etc.) super block-scytek 8 min
Primary Antibody (Diluent/Dilution/Incubation time, Wash step) ab47003 1:200,24590 1:100 in pbs+1%BSA for 1 hr RT,wash in tween 20 0.05% in PBS 3 times for 5 min while shaking
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
goat anti-rabbit Alexa Fluor 488 (Jackson Immuno Research Laboratories, Inc.), Goat Anti-Mouse Alexa Fluor 594 (Jackson Immuno Research Laboratories, Inc.), both 1:500
Detection method confocal
Positive and negative controls used (please specify) non, but secondary antibodies were in exact prior use with great specificity, both abcam antibodies were stained not together to generate good results
Optimization attempts (problem solving):none
How many times have you tried the IHC? twice
Have you run a "No Primary" control?
secondary antibodies were in exact prior use with great specificity
Do you obtain the same results every time? some pictures you don’t see much cross reactivity, some you see

Read More

Abcam community

Verified customer

Asked on Dec 20 2012

Answer

Thank you for contacting us.
I appreciate the time taken to submit protocol information. The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would also appreciate if you could please provide the order and batch details.
Reviewing this case, I would like to offer some suggestions to help optimise the results from these two antibodies in ICC/IF:
Cell fixation should be done under cold conditions. Using ice cold methanol, and optimizing it with other fixatives, such as 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperature.
For antibody ab24590 permeabilization is not required as the target protein is expressed in the membrane. For ab47003 I would recommend increasing the permeabilization conditions, by using more concentrated Tween 20 or 0.25% Triton X-100.
The unspecific signal obtained may be due to unspecific binding of the antibody as there has not been any blocking of unspecific binding sites. This could be improved by using 3%BSA for 30 min / 1 hour at room temperature.
Also, in order to facilitate the antibody to reach the target, incubation overnight at 4C normally gives better results than 1 hour incubation.
I hope this information is helpful. Should the suggestions not improve the results, please do not hesitate to contact me again and I will try to provide further help.

Read More

Abcam Scientific Support

Answered on Dec 20 2012

Question

Our customer retested following your suggestion.
1.Permeabilization / Blocking agent : 3% BSA, 0.3M glycine, 0.1% Triton-X 100

2. Concentration ofprimary antibody: 1/400
3. Concentration of secondary antibody :1/50

But she said they still stained in nucleus and showed dotty not clear pattern.
Please check attached file.

Best regards

Read More

Abcam community

Verified customer

Asked on Jul 16 2012

Answer

Thank you for getting back to me with the new results obtained.

It seems from the image shared that the staining is much improved with the changes in the protocol. The nuclear staining is much improved and the striations observed would be expected in the staining of this protein. In order to ascertain if there is any non-specific staining I would suggest the following:

1. Carry out a "no primary" control. This would involve staining the cells with the secondary antibody only at the same dilution as used before (1/40), then measure the fluorescence using the same parameters used for the images shared with me, i.e. using the rhodamine filters. This will allow you to see exactly how much of the non-specific staining is derived from the secondary antibody binding to the cells non-specifically. Ifnon-specific binding of the secondary antibody is observed Iwould suggest reducing the concentration of secondary antibody used to 1/200 and 1/500 to see if the results improve.

2. What buffer is being used to dilute theprimary antibody? I would suggest using 3% BSA in PBST.

3. Do you know what staining you would expect to get in human mesenchymal stem cells? Do you have any references where similar staining has been performed? Do you have access to a good positive control such as cardiomyocytes?

I hope these suggestions will be of help.


I look forward to receiving your reply.

Read More

Abcam Scientific Support

Answered on Jul 16 2012

1-10 of 22 Abreviews or Q&A

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