• Product name
    Anti-Cardiac Troponin T antibody
    See all Cardiac Troponin T primary antibodies
  • Description
    Rabbit polyclonal to Cardiac Troponin T
  • Host species
  • Tested applications
    Suitable for: IHC-P, Sandwich ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Cat, DogDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human cardiac Troponin T.

    Read Abcam's proprietary immunogen policy (Peptide available as ab47005.)

  • Positive control
    • WB: Human fetal heart tissue lysate. IHC-P: Human heart tissue.
  • General notes




Our Abpromise guarantee covers the use of ab45932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) as Capture.
ICC/IF 1/400. Incubate for 1-2 hours.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).


  • Function
    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Tissue specificity
    Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
  • Involvement in disease
    Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
  • Sequence similarities
    Belongs to the troponin T family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac muscle troponin T antibody
    • Cardiomyopathy dilated 1D (autosomal dominant) antibody
    • Cardiomyopathy hypertrophic 2 antibody
    • CMD1D antibody
    • CMH2 antibody
    • CMPD2 antibody
    • cTnT antibody
    • LVNC6 antibody
    • MGC3889 antibody
    • OTTHUMP00000033864 antibody
    • OTTHUMP00000033865 antibody
    • OTTHUMP00000033866 antibody
    • OTTHUMP00000033867 antibody
    • OTTHUMP00000033870 antibody
    • OTTHUMP00000218095 antibody
    • RCM3 antibody
    • TNNT 2 antibody
    • TNNT2 antibody
    • TNNT2_HUMAN antibody
    • TnTc antibody
    • Troponin T cardiac muscle antibody
    • Troponin T type 2 (cardiac) antibody
    • Troponin T type 2 cardiac antibody
    • Troponin T, cardiac muscle antibody
    • Troponin T2 cardiac antibody
    see all


  • ab45932 staining cardiac Troponin T in human fetal cardiomyocytes by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.1% (PBS) saponin buffer and blocked in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 (0.1% saponin, 10% goat serum, 0.01% triton) and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (L+H), diluted 1/600 was used as secondary.

    See Abreview

  • ab45932 staining Cardiac Troponin T in rat cardiac cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in 3% paraformaldehyde. Samples were incubated with primary antibody (1/400 in diluent) for 1 hour at room temperature. A Cy3®-conjugated goat anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.

    See Abreview

  • IHC image of Cardiac Troponin T staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45932, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Anti-Cardiac Troponin T antibody (ab45932) at 1 µg/ml + Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

    Exposure time: 30 seconds
  • Standard Curve for Cardiac Troponin T (Analyte: Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.


This product has been referenced in:
  • Gorabi AM  et al. TBX18 transcription factor overexpression in human-induced pluripotent stem cells increases their differentiation into pacemaker-like cells. J Cell Physiol 234:1534-1546 (2019). Read more (PubMed: 30078203) »
  • Parveen S  et al. Amniotic membrane as novel scaffold for human iPSC-derived cardiomyogenesis. In Vitro Cell Dev Biol Anim 55:272-284 (2019). Read more (PubMed: 30798515) »
See all 29 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A


Expiration date: DD MM YYYY
I am very pleased to hear you would like to accept our offer and test ab45932 in Rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Human Cultured Cells (differentiated iPSC)
differentiated iPSC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 12 2016

Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Human pluripotent stem cell derived cardiomyocyte
Yes - saponin

Abcam user community

Verified customer

Submitted Nov 19 2014


Thank you for your enquiry. We are not aware of any references for the antibody ab45932 that include western blot data demonstrating recognition of intact and fragmented troponin T. There is a western blot of human heart tissue on the datasheet which shows detection of a band at about 40 kDa, in addition to several smaller, less intense bands which may represent isoforms of troponin T.

Read More


Thank you for contacting us.

The antibody ab45932 recognizes the amino acid sequence of the immunogen, so it should be capable of detecting protein fragments that contain this sequence, using the applications listed on the datasheet: western blotting, ELISA, etc..

All of the isoforms described at the UniProt entry for human cardiac troponin T contain the immunogen sequence. Here is the URL for the UniProt page:


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Immunocytochemistry/ Immunofluorescence
Rabbit Cell (RAT cardiac cells)
RAT cardiac cells
Yes - 0.5% Triton x-100 in 3% paraformaldehyde

Tova Volberg

Verified customer

Submitted Jul 17 2012


Thank you for your inquiry.

I am assuming your customer wrote an Abreview for ab45932 and rabbit?

If so, I can confirm that we received the Abreview (today 17.07.2012)and one of my colleagues is working on it.

Since the code was not mentioned in the submitted Abreview I added this for your convenience.

Please thank your customer for their valuable feedback. The code is now active and can be used to purchase further products.

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Immunocytochemistry/ Immunofluorescence
Rat Cell (neonatal cardiomyocyte)
neonatal cardiomyocyte
Yes - 0.1% TritonX-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Mrs. Jamie Soto

Verified customer

Submitted Jun 15 2012

Immunocytochemistry/ Immunofluorescence
Human Cell (Kardiomyocyten)
CytoPerm/Fix (BD)
Yes - WashPerm (BD)
Blocking step
WashPerm (BD) as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 07 2012


Thank you for calling Abcam earlier today.

Please let me know if the protocol tips we discussed prove successful or not.

The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

If there is anything else I can help you with, just let me know.

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1-10 of 13 Abreviews or Q&A

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