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Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
ICC/IF more details Unsuitable for:
Mouse, Rat, Horse, Human
Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.
WB: K562 cell lysate
ICC/IF: HeLa cells
IHC-P: Human ovary carcinoma tissue
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Predicted molecular weight: 25 kDa.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use a concentration of 100 - 250 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for IP.
Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.
Belongs to the casein kinase 2 subunit beta family.
Phosphorylated by alpha subunit.
Information by UniProt
Casein kinase 2 beta polypeptide antibody
Casein kinase II beta subunit antibody
Casein kinase II subunit beta antibody
Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.
Flow Cytometry - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10
cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) This image is courtesy of an anonymous Abreview
Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg
Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
Blocked for 30 minutes at 25°C.
Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"