Anti-Caspase-10/CASP-10 antibody (ab2012)
Key features and details
- Rabbit polyclonal to Caspase-10/CASP-10
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Caspase-10/CASP-10 antibody
See all Caspase-10/CASP-10 primary antibodies -
Description
Rabbit polyclonal to Caspase-10/CASP-10 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- WB: HeLa whole cell lysate. ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
DEAE-Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
-AMC and Asp-Glu-Val-Asp-
-AMC.
Isoform C is proteolytically inactive. -
Tissue specificity
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. -
Involvement in disease
Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
Post-translational
modificationsCleavage by granzyme B and autocatalytic activity generate the two active subunits.
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 843 Human
- Omim: 601762 Human
- SwissProt: Q92851 Human
- Unigene: 5353 Human
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Alternative names
- ALPS2 antibody
- Apoptotic protease Mch-4 antibody
- CASP 10 antibody
see all
Images
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All lanes : Anti-Caspase-10/CASP-10 antibody (ab2012) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Predicted band size: 59 kDa
Observed band size: 59 kDa -
Immunocytochemistry/Immunofluorescence of HeLa cells labeling Caspase-10/CASP-10 with Anti-Caspase-10/CASP-10 antibody (ab2012) at 5μg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab2012 has been referenced in 3 publications.
- Langford MP et al. Multiple caspases mediate acute renal cell apoptosis induced by bacterial cell wall components. Ren Fail 33:192-206 (2011). PubMed: 21332342
- Pagnan G et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB ; Human . PubMed: 19228726
- Green KA et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). PubMed: 15069074