Anti-Caspase-12 antibody (ab62484)
Key features and details
- Rabbit polyclonal to Caspase-12
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-12 antibody
See all Caspase-12 primary antibodies -
Description
Rabbit polyclonal to Caspase-12 -
Host species
Rabbit -
Specificity
This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
Database link: O08736 -
Positive control
- WB: Human heart lysate. Rat dorsal root ganglion. IHC-P: Human and rat heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Caspase-12 Antibody is (Large)affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (6) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
IHC-P | (1) |
Use a concentration of 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates. |
IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. -
Tissue specificity
Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. - Information by UniProt
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Database links
- Entrez Gene: 100506742 Human
- Entrez Gene: 156117 Rat
- Omim: 608633 Human
- SwissProt: Q6UXS9 Human
- SwissProt: Q920D5 Rat
- Unigene: 476989 Human
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Alternative names
- CASP 12 antibody
- CASP-12 antibody
- Casp12 antibody
see all
Images
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Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml
All lanes : Human heart lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit IgG secondary antibody
Predicted band size: 46 kDa
Observed band size: 46 kDa -
Immunohistochemical analysis of paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml.
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All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution
Lane 1 : Rat dorsal root ganglion
Lane 2 : Treated rat dorsal root ganglion
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat -
Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (115)
ab62484 has been referenced in 115 publications.
- Desai VG et al. Doxorubicin-induced delayed-onset subclinical cardiotoxicity in mice. J Appl Toxicol 42:778-792 (2022). PubMed: 34668590
- Duan S et al. Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells. Ren Fail 44:126-136 (2022). PubMed: 35172689
- Meng S et al. CircAXL Knockdown Alleviates Aβ1-42-Induced Neurotoxicity in Alzheimer's Disease via Repressing PDE4A by Releasing miR-1306-5p. Neurochem Res 47:1707-1720 (2022). PubMed: 35229272
- Malik A et al. Interleukin-10 Mitigates Doxorubicin-Induced Endoplasmic Reticulum Stress as Well as Cardiomyopathy. Biomedicines 10:N/A (2022). PubMed: 35453640
- Zhang H et al. Rutaecarpine ameliorates lipopolysaccharide-induced BEAS-2B cell injury through inhibition of endoplasmic reticulum stress via activation of the AMPK/SIRT1 signaling pathway. Exp Ther Med 23:373 (2022). PubMed: 35495603