Recombinant Anti-Caspr antibody [EPR7828] - BSA and Azide free (ab248583)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7828] to Caspr - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Caspr antibody [EPR7828] - BSA and Azide free
See all Caspr primary antibodies -
Description
Rabbit monoclonal [EPR7828] to Caspr - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa (Boiled and Unboiled), SH-SY5Y (Boiled and Unboiled), Neuro-2a (Boiled and Unboiled), PC-12 (Boiled and Unboiled) IHC-P: Human cerebrum and Human astrocytoma tissue sections ICC/IF: Neuro-2a cells
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General notes
ab248583 is the carrier-free version of ab133634.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7828 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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WB |
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa).
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ICC/IF |
1/100.
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Notes |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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WB
1/1000. Detects a band of approximately 165 kDa (predicted molecular weight: 156 kDa). |
ICC/IF
1/100. |
Target
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Function
Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells. -
Tissue specificity
Predominantly expressed in brain. Weak expression detected in ovary, pancreas, colon, lung, heart, intestine and testis. -
Sequence similarities
Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8506 Human
- Entrez Gene: 53321 Mouse
- Entrez Gene: 84008 Rat
- Omim: 602346 Human
- SwissProt: P78357 Human
- SwissProt: O54991 Mouse
- SwissProt: P97846 Rat
- Unigene: 408730 Human
see all -
Alternative names
- Caspr antibody
- Caspr1 antibody
- CNTNAP antibody
see all
Images
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Lanes 1-7 : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution (Purified)
Lane 8 : Anti-Caspr antibody [EPR7828] (ab133634) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate boiled
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate boiled
Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate unboiled
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate boiled
Lane 6 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate unboiled
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate boiled
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?This data was developed using ab133634, the same antibody clone in a different buffer formulation.
The molecular weight is consistent with that has been described in the literature (PMID: 20610764).
We are unsure about the nature of extra bands.
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This data was developed using ab133634, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Caspr with Purified ab133634 at 1:2000 dilution (0.29 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. -
This data was developed using ab133634, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Caspr with Purified ab133634 at 1:2000 dilution (0.29 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. -
Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) cells labeling Caspr with purified ab133634 at 1/100 (5.7 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133634).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248583 has not yet been referenced specifically in any publications.