• Product name
    Anti-Catalase antibody [1A1]
    See all Catalase primary antibodies
  • Description
    Mouse monoclonal [1A1] to Catalase
  • Host species
  • Tested applications
    Suitable for: IP, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HeLa whole cell lysate. IF/ICC: HepG2 cell line.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the 



Our Abpromise guarantee covers the use of ab16771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 19659692
Flow Cyt Use 1µg for 106 cells.

(Also see PMID: 19659692)




ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



  • Function
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities
    Belongs to the catalase family.
  • Post-translational
    The N-terminus is blocked.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all


  • ICC/IF image of ab16771 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16771, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunoprecipitation analysis of HeLa cell lysates with ab16771.

    Lane  1: Input. Lane 2: Precipitates.

  • Overlay histogram showing HeLa cells stained with ab16771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Cai M  et al. Disruption of peroxisome function leads to metabolic stress, mTOR inhibition, and lethality in liver cancer cells. Cancer Lett 421:82-93 (2018). Read more (PubMed: 29458144) »
  • Geillon F  et al. Structure-function analysis of peroxisomal ATP-binding cassette transporters using chimeric dimers. J Biol Chem 289:24511-20 (2014). Read more (PubMed: 25043761) »

See all 4 Publications for this product

Customer reviews and Q&As

As shown on the product datasheet, https://www.abcam.com/ab16771, upon delivery we recommend toaliquot and store this product at -20°C. At -20ºC an antibody can be kept for 5 years. Avoid freeze / thaw cycles.

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Liver, Tonsil, Lung and pellets from cell lines:HE)
Liver, Tonsil, Lung and pellets from cell lines:HE
Antigen retrieval step

Abcam user community

Verified customer

Submitted Sep 28 2010

Thank you for getting back to me. I am sorry for the delay, I was waiting for a response from the source of this antiserum. I have been able to obtain a recommended protocol for IP using this antiserum. However, this is still yet to be tested for t...

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Thank you for getting back to me. I am sorry for any confusion. ab16771 IS capable of immunoprecipitation. This was confirmed by performing immunoprecipitation followed by western blotting. However, immunoblotting detection was performed under DENA...

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Thank you for your enquiry. ab16771 has been shown to immunoprecipitate Catalase and to cross react against rat. Furthermore ab1877 and ab16772 have also been shown to immunoprecipitate Catalase in rat. Unfortunately all three antisera are yet to d...

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