For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that utilizes the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
Visit our FAQs page for tips and troubleshooting.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
|CS Cell Lysis Buffer||WM||1 x 25ml|
|CS Inhibitor (1 mM)||Red||1 x 20µl|
|CS Reaction Buffer||NM||1 x 5ml|
|CS Substrate Ac-VVR-AFC (10 mM)||Brown||1 x 200µl|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"