• Product name
    Anti-Cdc25A (phospho S76) antibody
    See all Cdc25A primary antibodies
  • Description
    Rabbit polyclonal to Cdc25A (phospho S76)
  • Host species
  • Specificity
    ab75743 detects endogenous levels of Cdc25A only when phosphorylated at serine 76.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Cdc25A around the phosphorylation site of serine 76 (M-G-SP-S-E).

  • Positive control
    • Human breast carcinoma tissue; extracts from A2780 cells untreated or treated with UV.



Our Abpromise guarantee covers the use of ab75743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100.


  • Function
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
  • Sequence similarities
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Domain
    The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
  • Post-translational
    Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 25a antibody
    • CDC25A antibody
    • CDC25A2 antibody
    • CDC25A2 CAG isoform antibody
    • Cell division cycle 25 homolog A (S. pombe) antibody
    • Cell division cycle 25A antibody
    • Cell division cycle 25A isoform a antibody
    • Cell division cycle 25A isoform b antibody
    • D9Ertd393e antibody
    • Dual specificity phosphatase Cdc25A antibody
    • M phase inducer phosphatase 1 antibody
    • M-phase inducer phosphatase 1 antibody
    • MGC115549 antibody
    • MPIP1_HUMAN antibody
    see all


  • ab75743, at a 1/50 dilution, staining Cdc25A in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.
  • All lanes : Anti-Cdc25A (phospho S76) antibody (ab75743) at 1/500 dilution

    Lane 1 : A2780 cell extracts, untreated
    Lane 2 : A2780 cell extracts, treated with UV

    Predicted band size: 59 kDa
    Observed band size: 59 kDa
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for your reply. I am sorry that the antibodies are still not producing good results in WB. We do not currently have new lots available for either product, so I have issued you a refund (Credit Note: ***). To redeem this refund, please have your purchasing agent contact our accounting department at us.credits@abcam.com. Please let me know if you have any further questions.

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Thank you for contacting us. I am sorry that these antibodies are giving weak signal in your samples.

For Cyclin B2, I would expect ab18250 to give good staining using the protocol you sent, however this protein is not very highly expressed in normal or cancerous liver. You may find better results testing a positive control such as bone marrow or HeLa cells.

The antibody ab75742 is phospho-specific, so I would not recommend using milk as a blocking agent. Milk contains endogenous phosphatases which can cleave the phosphorylations of interest and results in significantly reduced signal. Switching to BSA may help to improve the strength of your band. Have you done any treatment as a positive control to ensure that this particular phosphorylation is expressed?

I hope this helps, if not, please let me know and I will be happy to help you further.

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