Anti-Centaurin alpha 1 antibody (ab27476)
Key features and details
- Goat polyclonal to Centaurin alpha 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Centaurin alpha 1 antibody
See all Centaurin alpha 1 primary antibodies -
Description
Goat polyclonal to Centaurin alpha 1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Synthetic peptide:
CQEYAVEAHFKHKP
, corresponding to amino acids 362-374 of Human Centaurin alpha 1. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
Target
-
Relevance
The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP. -
Cellular localization
Cytoplasmic, Nuclear and Plasma membrane -
Database links
- Entrez Gene: 11033 Human
- Entrez Gene: 231821 Mouse
- Entrez Gene: 397453 Pig
- Entrez Gene: 171097 Rat
- Omim: 608114 Human
- SwissProt: O75689 Human
-
Alternative names
- ArfGAP with dual PH domains 1 antibody
- CENTA 1 antibody
- CENTA1 antibody
see all
Images
-
All lanes : Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Pig brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
-
IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab27476 has been referenced in 4 publications.
- Gutiérrez Y et al. KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling. J Cell Biol 220:N/A (2021). PubMed: 33999113
- Van Duzer A et al. ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival. Life Sci Alliance 2:N/A (2019). PubMed: 31792062
- Szatmari EM et al. Centaurin-a1-Ras-Elk-1 signaling at mitochondria mediates ß-amyloid-induced synaptic dysfunction. J Neurosci 33:5367-74 (2013). WB ; Rat . PubMed: 23516302
- Moore CD et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P ; Human, Rat . PubMed: 17635995