Anti-CerS2 antibody (ab176709)
Key features and details
- Rabbit polyclonal to CerS2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CerS2 antibody
See all CerS2 primary antibodies -
Description
Rabbit polyclonal to CerS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CerS2 aa 300 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_071358.1
Database link: Q96G23 -
Positive control
- HeLa, Jurkat and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
pH: 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Suppresses the growth of cancer cells. May be involved in sphingolipid synthesis. -
Tissue specificity
Expressed in kidney, liver, brain, heart, placenta and lung. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain. -
Cellular localization
Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 29956 Human
- Omim: 606920 Human
- SwissProt: Q96G23 Human
- Unigene: 741232 Human
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Alternative names
- Ceramide synthase 2 antibody
- CerS 2 antibody
- CerS2 antibody
see all
Images
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All lanes : Anti-CerS2 antibody (ab176709) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 3 minutes -
CerS2 was immunoprecipitated from 1 mg HeLa whole cell lysate using a rabbit anti-CerS2 antibody which recognizes an upstream epitope (Lane 1), ab176709 at 6 μg/mg lysate (Lane 2) or control IgG (Lane 3) and then 20% of the IP was loaded for western blot and blotted using ab176709 at 1 μg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab176709 has been referenced in 2 publications.
- Phillips GR et al. The long and the short of Huntington's disease: how the sphingolipid profile is shifted in the caudate of advanced clinical cases. Brain Commun 4:fcab303 (2022). PubMed: 35169703
- Shao X et al. Hsa-miR-221-3p promotes proliferation and migration in HER2-positive breast cancer cells by targeting LASS2 and MBD2. Histol Histopathol 37:1099-1112 (2022). PubMed: 35734966