• Product name
    ChIP Kit - One Step
    See all ChIP Kit kits
  • Assay type
  • Assay time
    5h 00m
  • Product overview

    Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.

    Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.

  • Notes


    The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

    If additional control primers are needed, they are available for purchase as ab171517 (reverse primer) and ab171518 (forward primer).

  • Tested applications
    Suitable for: ChIPmore details


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 48 tests 96 tests
    10X Wash Buffer 1 x 10ml 1 x 20ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    8-Well Strip Caps 6 units 12 units
    96-Well PCR Plate 1 unit 1 unit
    Adhesive Covering Film 1 unit 2 units
    Anti-RNA Polymerase II (1 mg/ml) 1 x 8µl 1 x 16µl
    ChIP Buffer 1 x 6ml 1 x 12ml
    DNA Release Buffer ECP3 1 x 14ml 1 x 28ml
    GAPDH Primer - Forward (20 µM) 1 x 8µl 1 x 16µl
    GAPDH Primer - Reverse (20 µM) 1 x 8µl 1 x 16µl
    Non-Immune IgG (1 mg/ml) 1 x 10µl 1 x 20µl
    Proteinase K (10 mg/mL) 1 x 55µl 1 x 110µl
    Sonication Frame 1 unit 1 unit
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab117138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a different species, GAPDH primers for that species will need to be acquired.


  • Chromatin was prepared from HeLa cells following Abcam's X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. ChIP was performed with ab8580 (Histone H3 tri-methyl K4) using ab117138. No antibody was added to the blank (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primes and probes are located in the first Kb of the transcribed region.

  • The analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit - One Step, with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.



This product has been referenced in:
  • Lu R  et al. COPS5 amplification and overexpression confers tamoxifen-resistance in ERa-positive breast cancer by degradation of NCoR. Nat Commun 7:12044 (2016). ChIP ; Human . Read more (PubMed: 27375289) »
  • Mercado-Pimentel ME  et al. The S100P/RAGE signaling pathway regulates expression of microRNA-21 in colon cancer cells. FEBS Lett 589:2388-93 (2015). Functional Studies ; Rat . Read more (PubMed: 26193421) »

See all 4 Publications for this product

Customer reviews and Q&As

la concentration de la chromatine peut être mesurée par Bradford ou par un assai BCA.

Notez que nous recommandons d’utiliser entre 5-10 ug de chromatine ce qui correspond à ˜ 0.5 to 1 x 10^6 cellules (la chromatine es...

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The wells of ab117138 are coated with protein A/G to bind to the antibody(s) that you use. Multiple antibodies can be used in the same well. However, multiple targeted proteins will be captured, which may make the downstream application and analysis di...

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The size can be checked by directly running the sheared chromatin as seen in the attached file. If DNA will be purified. The following quick method may be useful: a) Use 10 uL sample and add 40 uL H2O b) Reverse cross-link by adding 2 uL of 5 M ...

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In general, the chromatin is easy to be sonicated to 200-1000 bps. If the chromatin extracts can not be broken down fully by sonication, it may be due to insufficient time length, power intensity, foaming of chromatin solution during sonication etc. th...

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1. We tested this kit with human samples, but it should also work fine with mouse samples.

2. the primers included in the kit is only for human GAPDH. The following are the suggested primers specific for mouse GAPDH:

F: 5'-...

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Hello and I apologize for the late reply.

1. The strips are the same for the two
2. The concentration of chromatin can be measured by
regular protein quantification methods. Histone protein :DNA ratio is
about 2:1. <...

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L'anti-RNA pol II fournie dans le kit ab117138 est un monoclonal de souris, clone [CTD4H8] qui reconnait la partie C-terminale de toutes les formes de la polymerase (phosphorylée et non-phosphorylée).

J'espère qu...

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Thank you for your reply. I am glad to hear your are able to get the correct band.

Cross linking should not interfere with the IP; however cross-linking is a time-critical procedure. Cross-linking should generally only be carried out for a f...

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Thank you for contacting us. I am sorry to hear that you have been experiencing issues when in ChIP using this product. According to the Uniprot information for this protein (http://www.uniprot.org/uniprot/Q80TJ7)this product may recognize the ~1...

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Thank you for contacting us and your interest in our products.

The ChIP kit which your customer is interested in used the protein A beads for isolation and would therefore not be suitable for use with a goat polyclonal antibody. We do however...

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1-10 of 18 Abreviews or Q&A


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