Anti-Chemerin antibody (ab72965)
Key features and details
- Mouse polyclonal to Chemerin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chemerin antibody
See all Chemerin primary antibodies -
Description
Mouse polyclonal to Chemerin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Chemerin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Matched antibody pairs
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa. |
Target
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Tissue specificity
Highly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5919 Human
- Omim: 601973 Human
- SwissProt: Q99969 Human
- Unigene: 647064 Human
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Alternative names
- HP10433 antibody
- RAR responsive protein TIG2 antibody
- RAR-responsive protein TIG2 antibody
see all
Images
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All lanes : Anti-Chemerin antibody (ab72965) at 1/500 dilution
Lane 1 : Chemerin transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 19 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab72965 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab72965 has been referenced in 9 publications.
- Kasacka I et al. Immunohistochemical identification and assessment of the location of leptin, visfatin and chemerin in the liver of men with different body mass index. BMC Gastroenterol 22:233 (2022). PubMed: 35549673
- Chen S et al. Chemerin-9 Attenuates Experimental Abdominal Aortic Aneurysm Formation in ApoE-/- Mice. J Oncol 2021:6629204 (2021). PubMed: 33953746
- Haberl EM et al. Chemerin Is Induced in Non-Alcoholic Fatty Liver Disease and Hepatitis B-Related Hepatocellular Carcinoma. Cancers (Basel) 12:N/A (2020). PubMed: 33066325
- Li X et al. Elevated chemerin induces insulin resistance in human granulosa-lutein cells from polycystic ovary syndrome patients. FASEB J 33:11303-11313 (2019). PubMed: 31311314
- Liu-Chittenden Y et al. RARRES2 functions as a tumor suppressor by promoting ß-catenin phosphorylation/degradation and inhibiting p38 phosphorylation in adrenocortical carcinoma. Oncogene 36:3541-3552 (2017). PubMed: 28114280
- Liu-Chittenden Y et al. Serum RARRES2 Is a Prognostic Marker in Patients With Adrenocortical Carcinoma. J Clin Endocrinol Metab 101:3345-52 (2016). PubMed: 27336360
- Farsam V et al. Senescent fibroblast-derived Chemerin promotes squamous cell carcinoma migration. Oncotarget 7:83554-83569 (2016). ICC/IF ; Human . PubMed: 27907906
- Kennedy AJ et al. Chemerin Elicits Potent Constrictor Actions via Chemokine-Like Receptor 1 (CMKLR1), not G-Protein-Coupled Receptor 1 (GPR1), in Human and Rat Vasculature. J Am Heart Assoc 5:N/A (2016). PubMed: 27742615
- Sell H et al. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes 58:2731-40 (2009). WB ; Human . PubMed: 19720798