• Product name
    Chromatin Extraction Kit
    See all Chromatin Extraction kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    1h 00m
  • Product overview

    Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.




  • ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
  • Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A.

    Chromatin yield was 4 µg chromatin/106 cells.

    Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
    Image courtesy of anonymous Abreview



This product has been referenced in:
  • Zhu B  et al. The protective role of DOT1L in UV-induced melanomagenesis. Nat Commun 9:259 (2018). Read more (PubMed: 29343685) »
  • Lu R  et al. COPS5 amplification and overexpression confers tamoxifen-resistance in ERa-positive breast cancer by degradation of NCoR. Nat Commun 7:12044 (2016). Functional Studies ; Human . Read more (PubMed: 27375289) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your inquiry.

We have two Nuclear Extraction Kits in our catalog at the moment:


https://www.abcam.com/index.html?datasheet=113474 (or use the following: https://www.abcam.com/index.html?datasheet=113...

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Under these conditions it should be fine to reduce the amount of working extraction buffer so that the chromatin is less dilute however sonication using a water bath sonicator may be necessary to improve rupture of the nuclear membrane and release of c...

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In general the chromatin fraction is 90% pure but it also depends on additional factors such as the cell/tissue type, and the chromatin extraction procedure itself such as how effectively the cytoplasmic fraction is removed.

Likewise, t...

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I can confirm that the chromatin fraction is 90% pure in general but it also depends on additional factors such as the cell/tissue type, and the chromatin extraction itself such as how effectively the cytoplasmic fraction is removed.

The extraction buffer of this kit contains a low concentration of EDTA which should not significantly affect combination use of micrococcal nuclease with this kit as Ca++ concentration is 5 mM.

The size can be checked by directly running the sheared chromatin as seen in the attached file. If DNA will be purified. The following quick method may be useful: a) Use 10 uL sample and add 40 uL H2O b) Reverse cross-link by adding 2 uL of 5 M ...

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In general, the chromatin is easy to be sonicated to 200-1000 bps. If the chromatin extracts can not be broken down fully by sonication, it may be due to insufficient time length, power intensity, foaming of chromatin solution during sonication etc. th...

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I hope the sonication step is helpful. I look forward to hearing how things go!

In general, this kit could generate about 4 ug/1 million cells of chromatin or 2 ug of DNA (histone:DNA in chromatin is about 1.8-2 :1). Based on the described conditions by the customer, the relatively low yield of DNA seems mainly due to that nucleus...

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Thank you for your enquiry. My colleague Karen asked me to follow up with you.

How has it been determined that the chromatin is in the pellet rather than the supernatant?

Thanks in advance for the additional information!

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