Recombinant PE Anti-Chromogranin A antibody [EP1030Y] (ab213341)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP1030Y] to Chromogranin A
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Chromogranin A antibody [EP1030Y]
See all Chromogranin A primary antibodies -
Description
PE Rabbit monoclonal [EP1030Y] to Chromogranin A -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra)ometry: SHSY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1030Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
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Form
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
Alternative names
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
Images
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Overlay histogram showing SH-SY5Y cells stained with ab213341 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213341, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in SH-SY5Y cells fixed with 4% formaldehyde (10min), permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab213341 has not yet been referenced specifically in any publications.