Anti-Citrulline antibody (ab100932)
Key features and details
- Rabbit polyclonal to Citrulline
- Suitable for: ELISA, ICC
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Citrulline antibody
See all Citrulline primary antibodies -
Description
Rabbit polyclonal to Citrulline -
Host species
Rabbit -
Specificity
ab100932 reacts specifically with intrapeptidic citrulline independently of the amino acid sequence. It does not react with free citrulline, ornithine or arginine. Citrulline is an un-natural amino-acid and its expression is very low. Factors limiting the detection are the amount of endogenous citrulline within a protein and the accessibility of the antibody to these sites. -
Tested applications
Suitable for: ELISA, ICCmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Citrulline conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
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Positive control
- Hypercitrullinated Histones induced in HL60 cells by DMSO +calcium +calcium ionophore A23187
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC | (1) |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Target
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Relevance
The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
Images
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ELISA data showing the high immuno-reactivity of ab100932 against citrulline due to the high amounts of citrulline conjugated to BSA.
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ELISA data demonstrating the specificity of ab100932 to citrulline naturally present in histones (endogenous citrulline).
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Immunocytochemical analysis of Human buccal mucosal cells, staining Citrulline with ab100932.
Cells were fixed with acetone followed by 2% glutaraldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 15 hours at 4°C. An undiluted biotinylated anti-rabbit polyclonal IgG was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab100932 has been referenced in 13 publications.
- Chen Y et al. Characterization of Monoclonal Antibodies Recognizing Citrulline-Modified Residues. Front Immunol 13:849779 (2022). PubMed: 35359951
- Thålin C et al. Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma. J Thromb Haemost 18:2732-2743 (2020). PubMed: 32654410
- Fonseca Z et al. Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation. J Leukoc Biol 105:1167-1181 (2019). PubMed: 30913315
- Wiersma VR et al. Galectin-9 Is a Possible Promoter of Immunopathology in Rheumatoid Arthritis by Activation of Peptidyl Arginine Deiminase 4 (PAD-4) in Granulocytes. Int J Mol Sci 20:N/A (2019). PubMed: 31430907
- Verheul MK et al. Pitfalls in the detection of citrullination and carbamylation. Autoimmun Rev 17:136-141 (2018). PubMed: 29203292