Recombinant Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12680(2)] to Claudin 8
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Claudin 8 antibody [EPR12680(2)]
See all Claudin 8 primary antibodies -
Description
Rabbit monoclonal [EPR12680(2)] to Claudin 8 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human colon and Human colonic adenocarcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12680(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space. -
Tissue specificity
Expressed in the epididymis, mainly in the caput segment. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells. - Information by UniProt
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Database links
- Entrez Gene: 9073 Human
- Entrez Gene: 54420 Mouse
- Entrez Gene: 304124 Rat
- Omim: 611231 Human
- SwissProt: P56748 Human
- SwissProt: Q9Z260 Mouse
- Unigene: 162209 Human
- Unigene: 25836 Mouse
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Alternative names
- Claudin 8 antibody
- Claudin-8 antibody
- CLD8_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Claudin 8 with ab183738 at 1/1000 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Claudin 8 with ab183738 at 1/1000 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab183738 has been referenced in 7 publications.
- Čužić S et al. Claudins: Beyond Tight Junctions in Human IBD and Murine Models. Front Pharmacol 12:682614 (2021). PubMed: 34867313
- Ishihara H et al. Genetic and epigenetic profiling indicates the proximal tubule origin of renal cancers in end-stage renal disease. Cancer Sci 111:4276-4287 (2020). PubMed: 32860304
- Zhou S et al. Identification of claudin-1, -3, -7 and -8 as prognostic markers in human laryngeal carcinoma. Mol Med Rep 20:393-400 (2019). PubMed: 31115553
- Jia H et al. Identification of claudin-2, -6, -11 and -14 as prognostic markers in human breast carcinoma. Int J Clin Exp Pathol 12:2195-2204 (2019). PubMed: 31934042
- Zhang X et al. Differences in the expression profiles of claudin proteins in human nasopharyngeal carcinoma compared with non-neoplastic mucosa. Diagn Pathol 13:11 (2018). PubMed: 29402318
- Yang L et al. Differences in the expression profiles of claudin proteins in human gastric carcinoma compared with non-neoplastic mucosa. Mol Med Rep 18:1271-1278 (2018). PubMed: 29901188
- Ashikari D et al. CLDN8, an androgen-regulated gene, promotes prostate cancer cell proliferation and migration. Cancer Sci 108:1386-1393 (2017). Human . PubMed: 28474805