Immunoprecipitation abreview for Anti-Cleaved PARP1 antibody [E51]

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Abreviews
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Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
U2OS cells

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Lysis buffer
Concentration
10 µg/mg lysate

Western Blot antibody

Dilution
1/1000
Name

Additional data

Additional Notes
500 ug of whole cell lysates from U2OS cells were incubated with 5 ug of antibody for 3 hours at 4 degree. Then, protein A/G magnetic beads were added and incubated at 4 degree for 2 hours.

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Submitted Mar 06 2018

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