Anti-Cofilin antibody (ab29038)
Key features and details
- Rabbit polyclonal to Cofilin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cofilin antibody
See all Cofilin-1 primary antibodies -
Description
Rabbit polyclonal to Cofilin -
Host species
Rabbit -
Specificity
ab29038 recognises Cofilin 1. The immunogen sequence has only 3 amino acid differences from human Cofilin 2. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to amino acid residues at the N terminus of human Cofilin 1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab29038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 18 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 18 kDa. |
Target
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Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
Tissue specificity
Widely distributed in various tissues. -
Sequence similarities
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
Post-translational
modificationsPhosphorylated on Ser-3 in resting cells. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
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Database links
- Entrez Gene: 1072 Human
- Omim: 601442 Human
- SwissProt: P23528 Human
- Unigene: 170622 Human
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Alternative names
- 18 kDa phosphoprotein antibody
- CFL 1 antibody
- CFL antibody
see all
Images
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Western blot of jurkat cells expressing cofilin 1. Blots were treated with (lanes 2, 3, 5 & 6) or without (lanes 1 & 4) lambda phosphatase for 1 hr at 37 degrees C. In lanes 3 & 6, phosphatase treatments were performed in the presence of phospho-cofilin 1 (Ser-3) peptide. Blots were probed with anti-phospho-Cofilin 1 (Ser-3) (lanes 1-3;) or anti-Cofilin 1 (lanes 4-6; ab29038). Phosphatase treatment removes Ser-3 phosphorylation of Cofilin, but this phosphatase activity is reduced in the presence of peptide.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab29038 has been referenced in 3 publications.
- Zhou T et al. Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells. Mol Med Rep 13:4414-20 (2016). PubMed: 27052944
- Kamal AH et al. Investigation of adipocyte proteome during the differentiation of brown preadipocytes. J Proteomics 94:327-36 (2013). PubMed: 24129212
- Codreanu SG et al. Global analysis of protein damage by the lipid electrophile 4-hydroxy-2-nonenal. Mol Cell Proteomics 8:670-80 (2009). WB ; Human . PubMed: 19054759