Anti-Collagen I + Collagen III antibody (ab24135)
Key features and details
- Rabbit polyclonal to Collagen I + Collagen III
- Suitable for: IHC-Fr, ELISA
- Reacts with: Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Collagen I + Collagen III antibody
See all Collagen I + Collagen III primary antibodies -
Description
Rabbit polyclonal to Collagen I + Collagen III -
Host species
Rabbit -
Specificity
Specificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining. -
Tested applications
Suitable for: IHC-Fr, ELISAmore details -
Species reactivity
Reacts with: Pig -
Immunogen
Full length native collagen type I and III from pig skin.
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Positive control
- Pig kidney and skin. Cultured porcine cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 1ml of distilled water and add preservative if required. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 8.00
Constituents: 0.16% Sodium phosphate, 0.38% Sodium borate, 0.87% Sodium chloride, 1% Dextran, 1% Mannitol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Pooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/20.
|
|
ELISA |
1/3000.
|
Notes |
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IHC-Fr
1/20. |
ELISA
1/3000. |
Target
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Relevance
Collagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons. -
Cellular localization
Secreted -
Alternative names
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- COL1A1 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab24135 has been referenced in 1 publication.
- Luo Q et al. hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4. Mol Ther Nucleic Acids 24:591-609 (2021). PubMed: 33898108