Anti-Connexin 43 / GJA1 antibody [Connexin 43] (ab78055)
Key features and details
- Mouse monoclonal [Connexin 43] to Connexin 43 / GJA1
- Suitable for: IHC-Fr, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Connexin 43 / GJA1 antibody [Connexin 43]
See all Connexin 43 / GJA1 primary antibodies -
Description
Mouse monoclonal [Connexin 43] to Connexin 43 / GJA1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Connexin 43 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
Tissue specificity
Expressed in the heart and fetal cochlea. -
Involvement in disease
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 14609 Mouse
- SwissProt: P23242 Mouse
- Unigene: 378921 Mouse
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Alternative names
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab78055 has been referenced in 9 publications.
- Wei X et al. Hypoglycemia-Exacerbated Mitochondrial Connexin 43 Accumulation Aggravates Cardiac Dysfunction in Diabetic Cardiomyopathy. Front Cardiovasc Med 9:800185 (2022). PubMed: 35369285
- Jourdeuil K & Taneyhill LA The gap junction protein connexin 43 controls multiple aspects of cranial neural crest cell development. J Cell Sci 133:N/A (2020). PubMed: 31964703
- Zhang J et al. Suppression of Connexin 43 Leads to Strial Vascular Hyper-Permeability, Decrease in Endocochlear Potential, and Mild Hearing Loss. Front Physiol 11:974 (2020). PubMed: 32922309
- Xu T et al. MiR-218 regulated cardiomyocyte differentiation and migration in mouse embryonic stem cells by targeting PDGFRa. J Cell Biochem 120:4355-4365 (2019). PubMed: 30246400
- Tavora F et al. Quantitative Immunohistochemistry of Desmosomal Proteins (Plakoglobin, Desmoplakin and Plakophilin), Connexin-43, and N-cadherin in Arrhythmogenic Cardiomyopathy: An Autopsy Study. Open Cardiovasc Med J 7:28-35 (2013). PubMed: 23802019
- Gorbe A et al. Myoblast proliferation and syncytial fusion both depend on connexin43 function in transfected skeletal muscle primary cultures. Exp Cell Res 313:1135-48 (2007). PubMed: 17331498
- Pearson RA et al. Gap junctions modulate interkinetic nuclear movement in retinal progenitor cells. J Neurosci 25:10803-14 (2005). PubMed: 16291954
- Saitongdee P et al. Levels of gap junction proteins in coronary arterioles and aorta of hamsters exposed to the cold and during hibernation and arousal. J Histochem Cytochem 52:603-15 (2004). PubMed: 15100238
- Wright CS et al. Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation. Reproduction 121:77-88 (2001). PubMed: 11226030