Recombinant Anti-Cullin 3/CUL-3 antibody [EPR3195] (ab108407)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3195] to Cullin 3/CUL-3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cullin 3/CUL-3 antibody [EPR3195]
See all Cullin 3/CUL-3 primary antibodies -
Description
Rabbit monoclonal [EPR3195] to Cullin 3/CUL-3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y, PC12 and NIH 3T3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 89 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 89 kDa. |
Target
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Function
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the cullin family. -
Post-translational
modificationsNeddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. -
Cellular localization
Nucleus. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 8452 Human
- Entrez Gene: 26554 Mouse
- Entrez Gene: 301555 Rat
- Omim: 603136 Human
- SwissProt: Q13618 Human
- SwissProt: Q9JLV5 Mouse
- SwissProt: B5DF89 Rat
- Unigene: 372286 Human
see all -
Alternative names
- CUL 3 antibody
- Cul-3 antibody
- cul3 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab108407 has been referenced in 6 publications.
- Qiao C et al. PD173074 blocks G1/S transition via CUL3-mediated ubiquitin protease in HepG2 and Hep3B cells. PLoS One 15:e0234708 (2020). PubMed: 32555680
- Pallett MA et al. Vaccinia Virus BBK E3 Ligase Adaptor A55 Targets Importin-Dependent NF-?B Activation and Inhibits CD8+ T-Cell Memory. J Virol 93:N/A (2019). PubMed: 30814284
- Dong B et al. Detection of RACK1 and CTNNBL1-induced activation of mouse splenocytes using an immunoprecipitation-based technique. Mol Med Rep 16:7056-7063 (2017). PubMed: 28901482
- Cristini A et al. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res 44:1161-78 (2016). WB ; Human . PubMed: 26578593
- Zhuo X et al. Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis. J Cell Biol 210:727-35 (2015). PubMed: 26323689
- Lee J et al. Autophagy suppresses interleukin-1ß (IL-1ß) signaling by activation of p62 degradation via lysosomal and proteasomal pathways. J Biol Chem 287:4033-40 (2012). IP . PubMed: 22167182