Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected cytoplasmic & nuclear staining pattern and is enriched within G1 cells. MeOH fixed samples were also evaluated and produced a similar, albeit slightly weaker staining pattern.
Dr. Kirk Mcmanus
Submitted Jan 22 2014
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