Anti-Cystathionase/CTH antibody (ab125210)
Key features and details
- Rabbit polyclonal to Cystathionase/CTH
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cystathionase/CTH antibody
See all Cystathionase/CTH primary antibodies -
Description
Rabbit polyclonal to Cystathionase/CTH -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cystathionase/CTH aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P32929 -
Positive control
- IHC-P: Human liver tissue. WB: SMCC, HT1080, HeLa and U-87 MG cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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Function
Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. -
Pathway
Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2. -
Involvement in disease
Defects in CTH are the cause of cystathioninuria (CSTNU) [MIM:219500]. It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. -
Sequence similarities
Belongs to the trans-sulfuration enzymes family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1491 Human
- Omim: 607657 Human
- SwissProt: P32929 Human
- Unigene: 19904 Human
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Alternative names
- CGL_HUMAN antibody
- CTH antibody
- cystathionase (cystathionine gamma-lyase) antibody
see all
Images
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All lanes : Anti-Cystathionase/CTH antibody (ab125210) at 0.5 µg/ml
Lane 1 : SMMC-7721 (Human hepatocarcinoma cell line) whole cell lysate
Lane 2 : HT-1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Predicted band size: 45 kDa -
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Cystathionase/CTH with ab125210 at 0.5 μg/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab125210 has been referenced in 2 publications.
- Zou S et al. Age-related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats. Int J Urol 28:459-465 (2021). PubMed: 33403726
- Yu L et al. Hydrogen sulfide donor, NaHS, stimulates ANP secretion via the KATP channel and the NOS/sGC pathway in rat atria. Peptides N/A:N/A (2018). WB ; Rat . PubMed: 29684589