Product nameAnti-Cytochrome P450 Reductase antibody
See all Cytochrome P450 Reductase primary antibodies
DescriptionGoat polyclonal to Cytochrome P450 Reductase
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Monkey, Zebrafish, Gorilla
- Human Adrenal tissue; Human, Mouse and Rat Liver lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab166800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionThis enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Involvement in diseaseDefects in POR are the cause of adrenal hyperplasia variant type (AHV) [MIM:201750]; also known as Antley-Bixler syndrome-like phenotype with disordered steroidogenesis. AHV is a rare variant of congenital adrenal hyperplasia. It is an autosomal recessive disorder with apparent combined P450C17 and P450C21 deficiency. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can also present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.
Defects in POR are a cause of isolated disordered steroidogenesis (IDS) [MIM:201750].
Sequence similaritiesIn the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the ER membrane by its N-terminal hydrophobic region.
- Information by UniProt
- CPR antibody
- CYPOR antibody
- Cytochrome p450 oxidoreductase antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal tissue labeling Cytochrome P450 Reductase with ab166800 at 3.75 µg/ml.
Anti-Cytochrome P450 Reductase antibody (ab166800) at 0.1 µg/ml + Rat Liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour.
ab166800 has not yet been referenced specifically in any publications.