Anti-Cytochrome b5 antibody (ab69801)
Key features and details
- Rabbit polyclonal to Cytochrome b5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome b5 antibody
See all Cytochrome b5 primary antibodies -
Description
Rabbit polyclonal to Cytochrome b5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide derived from an internal region of Human Cytochrome b5.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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ICC/IF |
1/500 - 1/1000.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
ICC/IF
1/500 - 1/1000. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. -
Involvement in disease
Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. -
Sequence similarities
Belongs to the cytochrome b5 family.
Contains 1 cytochrome b5 heme-binding domain. -
Cellular localization
Cytoplasm and Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1528 Human
- Entrez Gene: 109672 Mouse
- Omim: 613218 Human
- SwissProt: P00167 Human
- SwissProt: P56395 Mouse
- Unigene: 465413 Human
- Unigene: 31018 Mouse
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Alternative names
- CYB 5 antibody
- CYB 5A antibody
- CYB5 antibody
see all
Images
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IHC image of ab69801 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69801, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-Cytochrome b5 antibody (ab69801) at 1/500 dilution
Lane 1 : extracts from A549 cells
Lane 2 : extracts from A549 cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa -
Left: NIH/3T3 cells stained with ab69801 at 1/500 dilution; Right: the antibody was pre-incubated with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab69801 has been referenced in 11 publications.
- Wang W et al. Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation. Onco Targets Ther 14:1007-1020 (2021). PubMed: 33603407
- Kumondai M et al. Heterologous expression of high-activity cytochrome P450 in mammalian cells. Sci Rep 10:14193 (2020). PubMed: 32843676
- Reed L et al. Application of hepatic cytochrome b5/P450 reductase null (HBRN) mice to study the role of cytochrome b5 in the cytochrome P450-mediated bioactivation of the anticancer drug ellipticine. Toxicol Appl Pharmacol 366:64-74 (2019). PubMed: 30685480
- Reed L et al. Cytochrome b 5 impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation: studies in hepatic cytochrome b 5 /P450 reductase null (HBRN) mice. Arch Toxicol 92:1625-1638 (2018). ELISA ; Mouse . PubMed: 29368147
- Stark K et al. CYB5A polymorphism increases androgens and reduces risk of rheumatoid arthritis in women. Arthritis Res Ther 17:56 (2015). IHC-P ; Human . PubMed: 25890314